中文摘要：

目的：应用MRMT-1大鼠乳腺癌骨转移性癌痛模型探讨环氧化酶．2选择性抑制剂塞来昔布的抗肿瘤及镇痛作用机制。方法：用MRMT-1大鼠乳腺癌细胞建立的49只大鼠癌痛模型。造模20天后将大鼠随机分为治疗组（25只）与对照组（24只），于塞来昔布干预后第21天取转移性骨肿瘤组织行COX-2、VEGF蛋白表达水平测定及机械性痛觉超敏测量。结果：治疗组转移性骨肿瘤组织中COX-2和VEGF的蛋白表达与对照组比较显著降低（4266．42±733．96VS7731．96±1361．41；6237．05±771．24VS7833．06±1053．66 P〈0．05）。机械性痛觉超敏反应全面减弱，差异有显著性意义（P〈0．05）。结论：塞来昔布可能通过下调COX-2、VEGF水平抑制肿瘤进展并具有良好的镇痛作用。

英文摘要：

Objective： To investigate the mechanism of cyclooxygenease-2 （COX-2） specific inhibitor celecoxib on the treatment of cancer pain in rats induced by MRMT-1 mammary gland carcinoma cells. Methods：The rat model of metastatic bone cancer pain was produced by intra-tibial inoculations of MRMT-1 rat mammary gland carcinoma cells in Sprague-Dawley rats. Twenty days after cancer pain model establishment, all 49 rats were randomly divided into control group （24 rats） and celecoxib treatment group （25 rats）. Twenty-one days after celecoxib treatment, COX-2 and VEGF protein expression in segments of metastatic bone cancer rats were detected by immunohistochemical SP method, respectively. Mechanical allodynia was measured by von Frey filaments test. Results： The expressions of COX-2 and VEGF proteins in celecoxib treatment group were significantly less than those in control group（4266.42 ±733.96 VS 7731.96 ±1361.41; 6237.05 ±771.24 VS 7833.06 ±1053.66, P 〈0.05）. The mechanical allodynia in celeeoxib treatment group was significantly less than that in control group （ P 〈 0. 05 ）. Conclusion： Celecoxib was an effective chemotherapeutic agent for inhibiting tumor progression and relieving cancer pain by down-regulation of COX-2 and VEGF expressions in the rat MRMT-1 mammary gland carcinoma cells model.