中文摘要：

目的建立高效液相色谱法同时测定板蓝根药材中尿嘧啶、次黄嘌呤、鸟苷、胸腺嘧啶、（R,S）-告依春5种成分的含量。方法 Hanbon Hedera C18色谱柱（4.6 mm×250 mm,5μm）;流动相乙腈（A）-水（B）,线性梯度洗脱,流速1.0 m L·min-1;检测波长254 nm,柱温30℃。结果尿嘧啶、次黄嘌呤、鸟苷、胸腺嘧啶、（R,S）-告依春的线性范围分别为1.97-39.40 mg·L^-1（r=0.999 9）,1.25-50.00 mg·L^-1（r=0.999 9）,0.25-10.40 mg·L^-1（r=0.999 6）,2.84-56.70 mg·L^-1（r=0.999 9）,0.72-28.80 mg·L^-1（r=0.999 8）;平均回收率分别为99.36%（RSD=0.91%）,99.79%（RSD=1.12%）,100.90%（RSD=1.71%）,98.67%（RSD=1.50%）,99.33%（RSD=0.94%）。结论该方法简便,重复性好,灵敏度高,结果准确可靠,可作为板蓝根质量控制的有效方法。

英文摘要：

Objective To establish a HPLC method for simultaneous determination of uracil,hypoxanthine,guanosine,thymine,and（ R,S）-goitrin in Isatidis Radix. Methods The determination was performed on a Hanbon Hedera C18column（ 4. 6 mm ×250 mm,5 μm）. The mobile phase consisted of acetonitrile（ A） and water（ B） with linear gradient elution at the flow rate of 1. 0 m L·min- 1. The column temperature was 30 ℃. Detection wavelength was 254 nm. Results The linear range of uracil,hypoxanthine,guanosine,thymine,and（ R,S）-goitrin was 1. 97- 39. 40 mg·L^-1（ r =0. 999 9）,1. 25-50. 00 mg·L^-1（ r =0. 999 9）,0. 25- 10. 40 mg·L^-1（ r =0. 999 6）,2. 84-56. 70 mg·L^-1（ r =0. 999 9）,and 0. 72-28. 80 mg·L^-1（ r =0. 999 8）,respectively. The average recovery was 99. 36%（ RSD = 0. 91%）,99. 79%（ RSD = 1. 12%）,100. 90%（ RSD = 1. 71%）,98. 67%（ RSD = 1. 50%）,and 99. 33%（ RSD = 0. 94%）,respectively. Conclusion The method is simple,accurate,reliable,reproducible and sensitive,which can be used as an effective method for quality control of Isatidis Radix.