中文摘要：

螺旋体 interrogans 是细螺旋体病的原因的代理人。在里面 L 的 vitro 生长。interrogans 要求 CO2 和包含二 acyl-CoA carboxylases 的一条部分 3-hydroxypropionate 小径被 genomic 分析建议吸收 CO2。候选人基因的任何一个集合 heterologously 在 Escherichia coli 共同表示能表明两 acetyl-CoA carboxylase (ACC ) 和 propionyl-CoA carboxylase (PCC ) 活动。尽管没能被净化， tri 子单元 holoenzyme (LA_2736-LA_2735 和 LA_3803 ) 基于它向 acetyl-CoA 的底层偏爱是指明的 ACC。部分净化的双性人子单元 holoenzyme (LA_2432-LA_2433 ) 比 acetyl-CoA，并且这样，指明了 PCC 的作为底层对 propionyl-CoA 举办一项更加更高的活动。本国的 polyacrylamide 胶化电气泳动显示这 PCC 有约 669 kDa 的一个分子的团，建议 44 第四级的结构和当模特儿的结构的相同和它的 carboxyltransferase 子单元( LA_2433 )的指导地点的 mutagenesis 分析显示在通常认为的底层绑定衣袋的底部定位的 A431 残余可以在底层特性决心起一个重要作用。transcriptomic 和 proteomic 数据显示涉及建议部分 3-hydroxypropionate 小径的酶除了 ACC/PCC 在 vivo 被表示，在另外的螺旋体种类的染色体的相应基因因此被重新注解。然而，在里面 vitro 在粗略的细胞摘录检测了 ACC 的特定的活动太低，不能在 EllinghausenMcCulloughJohnsonHarris 最小的媒介说明细菌的生长，进一步系统的分析被要求经由 anaplerotic CO2 揭开 gluconeogenesis 的机制在螺旋体的吸收种。

英文摘要：

Leptospira interrogans is the causative agent of leptospirosis. The in vitro growth of L. interrogans requires CO2 and a partial 3-hydroxypropionate pathway involving two aeyl-CoA carboxylases was suggested by genomic analysis to assimilate CO2. Either set of the candidate genes heterologously co-expressed in Escherichia coli was able to demonstrate both aeetyl-CoA carboxylase （ACC） and propionyi-CoA carboxylase （PCC） activities. The trisubunit holoenzyme （LA_2736-LA 2735 and LA 3803）, although failed to be purified, was designated ACC based on its substrate preference toward acetyl-CoA. The partially purified bi-subunit holoenzyme （LA_2432- LA_2433） has a considerably higher activity against pro- pionyl-CoA as the substrate than that of acetyl-CoA, and thus, designated PCC. Native polyacrylamide gel electro- phoresis indicated that this PCC has a molecular mass of around 669 kDa, suggesting an α4β4 quaternary structure and both structural homology modeling and site-directed mutagenesis analysis of its carboxyltransferase subunit （LA_2433） indicated that the A431 residue located at the bottom of the putative substrate binding pocket may play an important role in substrate specificity determination. Both transcriptomic and proteomic data indicated that enzymes involved in the suggested partial 3-hydroxypro- pionate pathway were expressed in vivo in addition to ACC/PCC and the homologous genes in genomes of other Leptospira species were re-annotated accordingly. However, as the in vitro detected specific activity of ACC in the crude cell extract was too low to account for the growth of the bacterium in Ellinghausen-McCuliough- Johnson-Harris minimal medium, further systematic analysis is required to unveil the mechanism of gluconeo- genesis via anaplerotic C02 assimilation in Leptospira species.