中文摘要：

目的研究细菌细胞壁成分MDP对口腔癌细胞Tca8113 COX-2/PGE2表达的影响。方法 RT-PCR检测NOD2、COX-2在Tca8113细胞的表达;不同浓度MDP刺激Tca8113细胞后,采用RT-qPCR、MTT、ELISA及趋化实验分别检测其对COX-2/PGE2表达、细胞增殖和细胞趋化的影响。结果①Tca8113细胞表达NOD2、COX-2 mRNA;MDP促进COX-2 mRNA表达;②与对照组比较,MDP刺激Tca8113后PGE2分泌、细胞增殖、趋化显著增加（P〈0.05）;与MDP组比较,MDP＋NS-398组PGE2分泌、细胞增殖、趋化显著降低（P〈0.05）;与NS-398组比较,MDP＋NS-398组细胞增殖仍然显著增强（P〈0.05）。结论 MDP促进肿瘤细胞趋化和增殖,上调口腔癌细胞COX-2/PGE2表达是其作用机制之一。

英文摘要：

【Objective】 To study the effect of muramyl acylating dipeptide（MDP） on the COX-2/PGE2 expression in oral cancer cells Tca8113.【Methods】 NOD2 and COX-2 expressions in oral cancer cells were detected by RT-PCR.The COX-2 mRNA expression,cell proliferation,tumor cell secretion of PGE2 and cell chemotaxis in Tca8113 cells after stimulated by MDP and/or NS-398 were evaluated by RT-qPCR,CCK-8,ELISA and cell chemotaxis assay,respectively.【Results】 NOD2 and COX-2 mRNA could be detected in Tca8113 cells.When compared with the control group,a significant enhancement in COX-2 expression was observed in the MDP group at different concentrations.The PGE2 secretion,cell proliferation and cell chemotaxis in the MDP group were also significantly increased（P〈0.05）.When compared with the MDP group,significant decreases in the PGE2 secretion,cell proliferation and cell chemotaxis were observed in the MDP plus NS-398 group（P〈0.05）.Moreover,the cell proliferation rather than PGE2 secretion and chemotaxis in the MDP plus NS-398 group were still significantly increased when compared with that in the NS-398 group（P〈0.05）.【Conclusion】 MDP can increase the proliferation and chemotaxis of oral cancer cells.The upregulated COX-2/PGE2 expression is one of the mechanisms.