中文摘要：

本研究旨在阐明FSH调节睾丸支持细胞增殖的机制。试验以2～3周龄的仔猪睾丸支持细胞为实验材料,采用体外培养模型,通过细胞数量检测、ELISA和Western blot等方法研究FSH对睾丸支持细胞的调节作用。试验结果如下：（1）在0～50ng·mL-1内,随着FSH浓度的增加,睾丸支持细胞数量呈增加的趋势（P〈0.05）;当浓度为50ng·mL-1时,FSH促增殖作用最明显;细胞内cAMP的浓度也随着FSH的浓度而增加（P〈0.05）;（2）FSH作用后5min内就激活了ERK1/2级联反应,在48h内,ERK1/2的激活有一定的周期性;加入ERK1/2的抑制剂PD98059和U0126明显的降低了FSH诱导的睾丸支持细胞的增殖和PCNA的表达（P〈0.05）;（3）加入FSH和Forskolin增强了细胞的增殖和ERK1/2的激活（P〈0.05）;而加入Rp-cAMP则明显降低了FSH诱导的细胞增殖和ERK1/2的激活（P〈0.05）;L-Ca2＋通道抑制剂Verapamil抑制了细胞的增殖和ERK1/2的激活;Rp-cAMP与Verapamil共同作用强于单独作用,表现出一定的协同效应（P〈0.05）。结果表明,FSH通过cAMP、Ca2＋激活ERK1/2,并通过ERK1/2调节PCNA的表达进而调节支持细胞的增殖。

英文摘要：

This experiment was conducted to study mechanisms of FSH regulating Sertoli cell proliferation.Sertoli cells of 2-3 week-old piglet were used as cell models,and cell viability,ELSIA and Western blot were used to explain the effect of FSH.The results were as follows：（1） From 0 to 50 ng·mL-1,with the increase of the concentration of FSH,Sertoli cell number showed an upward trend（P0.05）;at 50 ng·mL-1,effect of FSH on Sertoli cell proliferation was most significant;the concentration of intracellular cAMP also increased,following the increase of the concentration of FSH（P0.05）;（2） FSH begun to activate ERK1/2 cascade reaction 5 min after its affecting and the activating model had a periodicity;ERK1/2 inhibitor（PD98059 and U0126） could significantly inhibit the FSH-induced Sertoli cell proliferation and the expression of PCNA（proliferating cell nuclear antigen）（P0.05）;（3） Both of FSH and Forskolin could enhance cell proliferation and ERK1/2 activation（P0.05）,while Rp-cAMP and L-Ca 2＋ channel inhibitor（Verapamil） significantly both inhibited the FSH-induced cell proliferation and ERK1/2 activation（P0.05）;Rp-cAMP and Verapamil showed synergism effect（P0.05）.These results indicated that FSH could activate ERK1/2 via cAMP and Ca 2＋,which increased the expression of PCNA,and promoted Sertoli cell proliferation.