中文摘要：

RNA 干扰(RNAi ) ， posttranscriptional 基因 silencing 进程由对指向的抄本明确地补足的小双 stranded RNA 调停了，包括长期的骨髓内产生的白血病(电流型逻辑) 对各种各样的人的疾病在新奇治疗学的途径的开发广泛地被使用了。这里，我们在电流型逻辑房间线 K562 报导控制四圜素的 siRNA 的成功的构造。稳定地表示反向的控制四圜素的 transactivator (rtTA ) 的一根 K562 房间线被构造。四圜素应答的元素(TRE ) 集成于被用来驱使特定的 siRNA 指向新奇 cytokine 的 pBS/U6 的 RNA III 倡导者区域像受体的因素 3 (CRLF3 ) 基因。结果证明 rtTA 能认出 TRE 阻止调停 siRNA 的外长、内长的 CRLF3 基因压抑。而且， CRLF3-siRNA 调停了基因压抑能面对 doxycycline 以一种剂量依赖者方式被导致。因此，在 K562 房间的可诱导的 siRNAi 系统可能为对电流型逻辑的调停 RNAi 的治疗学的途径的学习是有用的。

英文摘要：

RNA interference （RNAi）, a posttranscriptional gene silencing process mediated by small doublestranded RNA specifically complementary to the targeted transcript, has been used extensively in the development of novel therapeutic approaches against various human diseases including chronic myelogenous leukemia （CML）. Here, we report the successful construction of a tetracycline-controlled siRNA in CML cell line K562. A K562 cell line stably expressing the reverse tetracycline-controlled transactivator （rtTA） was constructed. A tetracy- cline responsive element （TRE） was integrated into the RNA polymerase Ⅲ promoter region of pBS/U6 that was used to drive specific siRNA to target the novel cytokine receptor-like factor 3 （CRLF3） gene. The results show that rtTA was able to recognize the TRE to prevent siRNA-mediated exogenous and endogenous CRLF3 gene repressions. Moreover, CRLF3-siRNA mediated gene repression could be induced in a dose-dependent manner in the presence of doxycycline. Thus, the inducible siRNAi system in K562 cells might be useful for the study of RNAi-mediated therapeutic approaches against CML.