依据已发表的绵羊MTNR1α基因外显予2扩增引物序列。以内蒙古绒山羊基因组DNA为模板进行PCR扩增,得到824bp的基因序列,将扩增产物进行克隆测序后与GenBank数据库进行序列同源性比较分析。结果表明,绒山羊MTNR1α外显子2扩增序列与已发表的山羊同源性为99.2%,与绵羊、牛、人、鼠同源性分别为98.5%、96.8%、82.O%和78.4%,内蒙古绒山羊MNTR1α基因第2外显子氨基酸序列与已发表的山羊氨基酸序列的同源性为97.7%,与已发表的绵羊、牛、人和鼠的氨基酸同源性分别为94.1%、91.8%、82.2%、83.1%。
According to the nucleotide sequences of announced in sheep, primers were designed. The 824 bp fragment of exon 2 of MTNR1α gene was amplified by PCR in Inner Mongolian cashmere goat. The PCR products were purified and cloned. Then the recombinant plasmids were sequenced. By blasting with database of GenBank, homology of nncleotide sequence of exon2 MTNRla gene between goat, sheep, cow, human and mouse were 99.2%, 98.5%, 96.8%, 82.0% and 78.4%,respectively. The homology of amino acids nucleotide sequence of exon2 MTNR1α gene between goat, sheep, cow, human and mouse were 97.7%, 94.1%, 91.8%, 82.2% and 83.1%, respectively.