中文摘要：

目的：探讨姜黄素预处理对大鼠肢体缺血再灌注肺损伤时血红素加氧酶-1（HO-1）的影响。方法：建立大鼠肢体缺血2h再灌注3h肺损伤模型。60只大鼠随机等分为5组：假手术组（Sham）、姜黄素组（Cur）、缺血再灌注组（I／R）、姜黄素＋缺血再灌注组（Cur—I／R）和锌原卟啉IX（ZnPP）＋姜黄素＋缺血再灌注组（ZnPP-Cur—I／R）。ZnPP—Cur—I／R组于缺血前24h经腹腔注射ZnPP20mg／kg，Cur组、Cur-I／R组和ZnPP-Cur—I／R组分别于缺血前2h经腹腔注射姜黄素200mg／kg，其余各组给予等容量生理盐水。除Sham组和Cur组外，其余各组行肢体缺血再灌注。观察肺组织病理学变化，测定肺组织损伤评分、湿／干重比（W／D）、中性粒细胞（PMN）数目和HO-1mRNA及其蛋白表达的情况。结果：与Sham组比较，I／R组肺组织损伤较重，肺损伤评分、W／D及PMN计数升高，HO-1mRNA及其蛋白表达上调（P〈0．01）；与I／R组比较，Cur—I／R组肺组织损伤减轻，肺损伤评分、W／D及PMN计数降低，HO-1mRNA及其蛋白表达上调（P〈0．01）；与Cur—I／R组比较，ZnPP—Cur—I／R组肺组织损伤加重，肺损伤评分、W／D及PMN计数升高，HO-1mRNA及其蛋白表达下调（P〈0．01）。结论：姜黄素预处理可通过上调肺组织HO—1的表达减轻大鼠肢体缺血再灌注肺损伤。

英文摘要：

AIM. To investigate the effects of curcumin pretreatment on heine oxygenase-1 （HO-1） in lung injury induced by ischemia- reperfusion （I/R） of hind limbs in rats. METHODS. The model of lung injury was established by occlusion of bilateral femoral arteries for 2 h followed by 3 h reperfusion in rat. Sixty SD rats were randomly divided into five equal groups. Sham group, Curcumin group （Cur group）, I/R group, Curcumin-l-I/R group （Cur-I/R group）, Zinc protoporphyrin IX-l- Curcumin if- I/R group （ZnPP-Cur-I/R group）. ZnPP-Cur-I/R group received ZnPP 20 mg/kg via intraperitoneal injection 24 h before ischemia. Cur, Cur-I/R and ZnPP-Cur-I/R groups received curcumin 200 mg/kg respectively via intraperitoneal injection 2 h before ischemia. I/R of hind limbs was induced in three I/R groups. The lungs were removed for pathologic examination and determination of the lung Wet/dry weight ratio （W/D）, lung injury score and polymorphonuclear neutrophil （PMN） count. The expression of HO-1 mRNA and protein in lung tissues was detected using RT-PCR and Western blot analysis respectively. RESULTS. Alveolar edema, localized pul-monary atelectasis and large amount of PMN infiltration were found in I/R group and were ameliorated in Cur-I/R group. Lung injury score, W/D ratio and PMN count were increased in I/R group as compared 0.01）. Lung injury with Sham group （P 〈2 score, W/D ratio and PMN count were significantly decreased in Cur-I/R group as compared with I/R group （P（0.01）. HO-1 mRNA and protein expression was significantly up-regulated in Cur-I/R group than in Sham and I/R groups （P 〈 0.01）. Compared with Cur-I/R group, the beneficial effects of curcumin were abolished, lung injury score, W/ D ratio and PMN count were significantly increased, and expression of HO-1 mRNA and protein was down-regulated in ZnPP-Cur-I/R group （P 〈20. 01）. CONCLUSION： Curcumin pretreatment has protective effects against lung injury induced by I/R through up-regulation of lung tissues. of hind li