中文摘要：

目的：研究基因中抑制序列对离子通道基因表达的影响．方法：我们将hKv4．3基因的启动子和5’非翻译区的一段序列（＋2-＋160，S160）以不同的位置和方向克隆到报告质粒pβ-gal-Basievector中，瞬时表达，进行β—galactosidase相对活性分析．结果：S160对hKv4．3基因表达具有强烈的抑制作用．结论：抑制序列S160对hKv4．3基因的抑制功能具有位置特异性．

英文摘要：

Objective：To study the effect of repressor on ion channel gene expression. Methods：We cloned hKv4.3 promoter and the sequence（ ＋ 2 - ＋ 160, S160）of 5＇UTR of hKv4.3 gene into pβ-gal-Basic vector. Transient expression of the pβ-gal vector and analysis of relative activity of β-galactosidase were undergone. Results： S160 intensively repressed the expression of hKv4.3 gene. Conclusion： We found that the inhibition of S160 to hKv4.3 gene with positionspecificity.