中文摘要：

目的将在骨头髓 stromal 房间(BMSC ) 在氧化导致压力的损坏和 apoptosis 上调查效果和人参属 Notoginseng saponins (PNS ) 的可能的机制。方法 BMSC 被孤立并且从由坡度 centrifugation 与支持者方法相结合的密度的 2-month-old 新西兰兔子有教养。第三个经过房间被用于随后的实验。氧化应力被 H2O2 (0.1 mmol/L ) 在有教养的 BMSC 导致。BMSC 是有为在 H2O2 处理前的 4 h 的 25200 g/mL PNS 的 pretreated。BMSC 的增长用 MTT 试金被观察。作为早 osteoblastic 区别的一个索引，碱的磷酸酶(高山) 活动与一个高山试金工具包被决定。流动 cytometry 被用来由与 annexinV-FITC/propidium 碘化物染色观察 BMSC 的 apoptosis。氧化压力水平被反应的氧检验种类(ROS ) 试金。在 BMSC 的 Bax， Bcl-2 和 Caspase-3 的蛋白质表情被西方的弄污分析。结果 PNS 在增长和 H2O2 导致的 BMSC 的造骨细胞区别上有不同集中依赖者效果。100 g/mL 的 PNS 集中作为最佳的有效集中被决定。PNS 显著地从 41.91% ～ 14.67% 稀释了导致 H2O2 的 apoptosis 率(P < 0.01 ) 。PNS 显著地减少了 H2O2 导致的 ROS 水平(P < 0.01 ) 。而且，有 PNS 的预告的处理显著地颠倒了 Bcl-2 表示和 Caspase-3 表示的导致 H2O2 的抑制(P < 0.01 ) 。结论 PNS 通过清除在有教养的兔子 BMSC 在氧化导致压力的损坏和 apoptosis 上有保护的效果 ROS 并且调整 Bcl-2/Bax 小径。

英文摘要：

Objective： To investigate the effects and possible mechanism of Panax Notoginseng saponins （PNS） on oxidative stress-induced damage and apoptosis in bone marrow stromal cells （BMSCs）.Methods： BMSCs were isolated and cultured from 2-month-old New Zealand rabbits by the density gradient centrifugation combined with adherent method.The third passage cells were used for subsequent experiments.Oxidative stress was induced in cultured BMSCs by H2O2 （0.1 mmol/L）.BMSCs were pretreated with 25-200 μg/mL PNS for 4 h before H2O2 treatment.Proliferation of BMSCs was observed using MTT assay.Alkaline phosphatase （ALP） activity,as an index of early osteoblastic differentiation,was determined with an ALP assay kit.Flow cytometry was used to observe the apoptosis of BMSCs by staining with annexinV-FITC/ propidium iodide.Oxidative stress level was examined by reactive oxygen species （ROS） assay.The protein expressions of Bax,Bcl-2 and Caspase-3 in BMSCs were analyzed by Western blotting.Results： PNS had different concentrationdependent effects on proliferation and osteoblast differentiation of BMSCs induced by H2O2.A PNS concentration of 100 μg/mL was determined as the optimal effective concentration.PNS markedly attenuated H2O2-induced apoptosis rate from 41.91% to 14.67% （P〈0.01）.PNS significantly decreased ROS level induced by H2O2 （P〈0.01）.Furthermore,pretreatment with PNS significantly reversed H2O2-induced inhibition of Bcl-2 expression and augmentation of Bax and Caspase-3 expression （P〈0.01）.Conclusion： PNS had a protective effect on oxidative stress-induced damage and apoptosis in cultured rabbit BMSCs through scavenging ROS and regulating the Bcl-2/Bax pathway.