中文摘要：

ω干扰素（IFN-ω）是同IFN-α功能相近但抗原性不同的一类干扰素分子，它具有同IFN-α、IFN-γ以及肿瘤坏死因子α相似的抑制病毒复制的作用．采用5L发酵罐发酵表达IFN-ω，发酵培养液经超滤膜浓缩、SP层析柱梯度分离，得到糖基化的IFN-ω，用基质辅助激光解析电离飞行时间质谱（MALDITOF-TOFMS）和N端蛋白序列分析仪分析其分子质量和N端序列，用细胞病变抑制法测定活性．结果表明，诱导约48h时，目的蛋白表达量最高，通过SP梯度洗脱，可将糖基化和非糖基化的IFN-ω分离，质谱分析糖基化蛋白样品，质谱峰为糖基化蛋白特有的峰．纯化后糖基化的IFN-ω抗病毒活性为1．46×10^8U／mL．

英文摘要：

IFN -ω is a kind of interferon molecules,which has similar function hut different antigenicity to IFN-α. It can inhibit virus replication like IFN-α,IFN-γ and tumor necrosis factor alpha. 5 L fermenter was used to ferment and interferon-ω was expressed. Then,the glyeosylated interferon-ω was purified by a procedure stepped by ultrafiltration and gradient elution SP chromatography column. Its molecular weight and N amino acid sequence were analyzed by MALDI TOF TOF MS and the N protein sequence analy- zer,respectively. In addition,IFN-ω activity was determined by cell changes suppression method. The results showed that IFN-ω ex pression level reached to highest in induced 48 h. The glycosylated and unglycosylated IFN-ω could be separated by SP gradient elu- tion completely. The glycosylated IFN co showed a series of continuous peaks by mass spectrometry,which is the characteristic peak of glycosyl protein. The antiviral activity of the purified giycosylated IFN-ω was 1.46 ×10^8 U/mL.