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中文摘要：
目的在保持关节软骨表面湿润的情况下,进行纳米压痕实验,研究具有一定生物活性的关节软骨微结构的力学性能。方法通过实验的方法,评估冷镶嵌法和保湿法对保持软骨在体力学性能的优劣,并应用保湿法得到不同保护液下软骨微结构的力学性能。结果冷镶嵌法测得的软骨表层的弹性模量远大于保湿法测得的数值,蒸馏水保护下的软骨表层和深层的弹性模量明显高于壳多糖溶液和生理盐水保护下的软骨的弹性模量。结论保湿法更有利于保持生物材料在体时的力学性能和保持生物材料的生物活性,通过保湿法也可以证明壳多糖溶液及生理盐水均可有效保持软骨的力学性能。
英文摘要：
Objective To study mechanical properties of the microstructure of the articular cartilage with biological activity by nanoindentation tests under the condition of keeping the articular cartilage in water.Methods The different abilities to keep in situ mechanical properties of the articular cartilage with cold mosaic method or with moisturizing method were evaluated by experiments,and the mechanical properties of the microstructure of the articular cartilage in different kinds of solution used in moisturizing method were also obtained.Results The experiment showed that the elastic modulus on the cartilage surface layer with cold mosaic method was much higher than that with moisturizing method,and the elastic modulus on the cartilage surface and deep layer in chitosan and saline were much lower than that in distilled water.Conclusions The moisturizing method is better at preserving in situ mechanical properties and biological activity of the biological materials,and chitosan and saline are proved to have the effect of keeping mechanical properties of the cartilage used in moisturizing method.
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