中文摘要：

目的分离及鉴定贵州、广西、广东各地临床分离的马尔尼菲青霉（Penicillium marneffei，PM），通过微卫星基因多态性分析进行分子流行病学初步研究。方法从贵州、广西、广东地区临床病人标本中分离马尔尼菲青霉，通过形态学及ITS序列进行鉴定，运用多位点微卫星分型法（multilocus microsatellite typing，MLMT）和SPSS18．0软件对分离的马尔尼菲青霉进行基因多态性及其相关性分析。结果贵州、广西和广东共收集鉴定马尔尼菲青霉临床株50株，3个徽卫星位点PMMI、PMMII、PMMIII分别检测到29、9和8种等位基因，50株菌共分为47个微卫星型（PMMT）；聚类分析将贵州及广西、广东地区马尔尼菲青霉分为6大类（Clusterl-Cluster 6）。结论贵州、广西、广东地区的马尔尼菲青霉具有较高的基因多态性，我国南方地区相邻省份马尔尼菲青霉的种群基因结构与地域分布之间存在着一定的相关性。

英文摘要：

In order to study the genetic polymorphism and molecular epidemiology of clinical Penicilliurn marneffei strains from different areas, 50 clinical strains isolated from Guangxi, Guangdong and Guizhou provinces were identified by morphology and ITS sequencing method. Then 50 Penicillium marneffei strains were amplified with PMMI, PMMII and PM- MIII primers by PCR method, and the PCR products were sized by automated capillary electrophoresis. According to the allele size, the 50 strains were classified by Multilocus Microsatellite Typing （MLMT） method and software SPSS 18.0. The results of MLMT showed that the Penicillium marneffei microsatellite loci--PMMI, PMMII and PMMIII loci detected 29, 9 and 8 al leles, respectively. Of 47 genotypes （PMMT） recognized among the 50 clinical Penicilliurn rnarneffei isolated from Guangxi, Guangdong and Guizhou provinces showed high gene polymorphism and some kind of genetic relevance. We speculated that there are some kind of relation between Penicillium rnarneffei geographic component and its population genetic structure at part of neighborhood areas in South China.