中文摘要：

对高效液相色谱法测定DNA总甲基化水平的关键因素进行研究,即基因组DNA的提取及纯化和高效液相色谱条件的选择。结果表明：CTAB法Ⅰ提取和纯化效果优于CTAB法Ⅱ;较优高效液相色谱条件为：采用Diamonsil C18（2）（250 mm×4.6 mm,5μm）的色谱柱,以甲醇-10 mmol/L磷酸二氢钾（10-90,v/v）为流动相构成,流动相pH为4.7,流速为0.5 mL/min,柱温为30℃,紫外检测器波长为285 nm时,是分离胞嘧啶和5-甲基胞嘧啶的较优条件。以试验优化的DNA提取方法和HPLC色谱条件,基因组DNA水解液的胞嘧啶（C）和5-甲基胞嘧啶（5 mC）可得到较好的分离效果。

英文摘要：

This study examines the critical methodological factors in the HPLC analysis of DNA methylation.It included DNA extraction and HPLC conditions choice.The experimental results showed that CTABⅠwas superior to CTABⅡ.The better HPLC conditions were Diamonsil C18（2）（250 mm×4.6 mm,5 μm） column,mobile phase consisting of methanol-KH2PO4（10 mmol/L）（10：90,V/V） at a flow rate of 0.5 ml/min,the column temperature 30 ℃,pH 4.7 and the detection wavelength 285 nm. With the optimized method of DNA extraction and HPLC conditions,the better result was achieved in the chromatogram of C and 5 mC in genomic DNA hydrolyzate.