中文摘要：

目的：观察雌、雄激素对老龄大鼠颏舌肌细胞肌浆网（SR）Ca^2＋-ATP酶活性及其基因表达的影响，探索性激素影响上气道稳定性的机制。方法：将24只老龄雄性大鼠随机分为3组：老龄对照组、老龄雌激素组（肌注苯甲酸雌二醇，每次0．1mg／kg，每周2次，共4周）和老龄雄激素组（肌注丙酸睾酮，每次2．5mg／kg，每周2次，共4周）。采用定磷法检测颏舌肌SR Ca^2＋-ATP酶活性，荧光定量RT-PCR测定SR Ca^2＋-ATP酶mRNA表达的变化。结果：与对照组相比，老龄雌激素组颏舌肌SRCa^2＋-ATP酶活性[前者34．24±6．14μmolPi／（mg protein·hour），后者38．39±8．49μmol Pi／（mgprotein·hour）]及其mRNA表达水平（前者0．0297±0．0140，后者0．0314±0．0174）差异无统计学意义（P〉0．05）；老龄雄激素组SRCa^2＋-ATP酶活性[22．91±4．56μmol Pi／（mg protein·hour）]下降到对照组的67％（P〈0．01），其mRNA表达水平（0．0172±0．0086）亦明显下降（P〈0．05）。结论：雄激素可能通过降低颏舌肌SR Ca^2＋-ATP酶活性、抑制其mRNA的表达而影响老龄大鼠颏舌肌的功能；而雌激素没有此项作用。

英文摘要：

Objective： To investigate the effects of sex hormones on sarcoplasmic reticulum （SR） Ca^2＋ -ATPase activity and gene expression in the genioglossal muscle of the aged rats, and to explore the possible mechanism related to the upper airway stability. Methods： Twenty four aged male rats were randomly divided into 3 groups： control group, estrogen group （intramuscular injection of estrogen 0.1 mg/ kg, twice a week） , androgen group （ intramuscular injection of androgen 2.5 mg/kg, twice a week）. Ca^2＋-ATPase activity was measured by detecting inorganic phosphorus ion, and SR Ca^2＋ -ATPase （SERCA） mRNA level was observed using fluorescence quantitative reverse transcription-polymerase chain reaction （FQ RT-PCR）. Results： Compared with the control group, there were no significant changes in SR Ca^2＋-ATPase activity [34.24 ± 6.14 μmol Pi/（mg protein · hour） vs 38.39 ± 8.49 μmol Pi/（mg protein · hour） ] and SERCA mRNA level （0.029 7 ± 0.014 0 vs 0.031 4 ±0.017 4 ） in estrogen group（P〉0.05） ; while in the androgen group, SR Ca^2＋-ATPase activity [22.91 ± 4.56 μmol Pi/（ mg protein · hour） ] was reduced to 67% （P 〈0.01 ）, and SERCA mRNA level （0.017 2 ± 0. 008 6） was also significantly reduced （P 〈 0.05 ）. Conclusion： Androgen may impair the function of the genioglossal muscle by depressing the SR Ca^2＋ -ATPase activity and the SERCA mRNA expression, while estrogen may not have this mechanism in the aged male rats.