中文摘要：

目的 探讨胚胎干细胞因子Sox2对神经母细胞瘤Ⅰ型细胞增殖分化的影响.方法 应用慢病毒载体介导RNA干扰处理Ⅰ型细胞BE（2）-C构建Sox2低表达细胞株,采用CCK-8活细胞数检测,分析Sox2对BE（2）-C细胞增殖能力的影响.维甲酸和5-溴2＆#39;-去氧尿甙干预BE（2）-C细胞诱导分化后采用Western blot检测细胞标志蛋白的表达差异,分析Sox2对BE（2）-C细胞分化能力的影响.结果 CCK-8活细胞数检测显示72 h时Sox2 shRNA组的细胞AV值（0.72＆#177;0.18）低于空白对照组（1.24＆#177;0.21）和阴性对照组（1.16＆#177;0.26）,差异有统计学意义（P＜0.05）.RA或BrdU诱导分化后Western blot检测细胞标志蛋白（Peripherin、NF-68、Vimentin、S-100）显示,经RA或BrdU诱导分化的Sox2 shRNA组细胞其标志蛋白表达量均高于经同种药物诱导的空白对照组（BE（2）-C）和阴性对照组（Sox2 shRNA空载体）细胞,差异有统计学意义（P＜0.05）.结论 Sox2促进神经母细胞瘤Ⅰ型细胞的增殖,但抑制其向低度恶性细胞分化,提示Sox2有望成为神经母细胞瘤基因治疗的新靶点.

英文摘要：

Objective To explore the effects of Sox2 on cell proliferation and differentiation of Ⅰtype neuroblastoma cell.Methods Sox2 mRNA knockdown BE （2）-C cell line was established by RNA interference via lentiviral vectors.Cell counting kit-8 （CCK-8） assay was applied to test the effect of Sox2 on the proliferation of BE（2）-C cell.Western blot was performed to detect the marker proteins after induced differentiation in order to analyze the effect of Sox2 on differentiation properties.Results The absorbance value （AV） of Sox2 shRNA cells（0.72 ＆#177; 0.18）was lower than BE（2）-C cells （1.24 ＆#177; 0.21）and shRNA control cells（1.16 ＆#177; 0.26）at 72 h in CCK-8 assay （P〈0.05）.Sox2 shRNA cells exhibited the elevated expression levels of marker proteins （Peripherin,NF-68,Vimentin ＆amp; S100）of N or S-type cell than BE（2）-C and shRNA control cells after induced differentiation （P〈0.05）.Conclusions Sox2 promotes cell proliferation and inhibits the differentiation of Ⅰ-type neuroblastoma cell.It may serve as a new target of gene therapy for neuroblastoma.