目的 研究稀硬木层孔菌(Phellinus robustus)子实体的化学成分。方法 采用溶剂法进行提取和萃取, 采用色谱和重结晶等方法进行分离纯化, 利用波谱法进行结构鉴定。结果 从中分离并鉴定了14个化合物, 分别为原儿茶醛(1), 原儿茶酸(2), 香豆素(3), 东莨菪苷(4), 22E, 24-麦角甾-7, 22-二烯-3β-醇(5), 5α, 8α-过氧麦角甾-6, 22-二烯-3β醇(6), (22E, 24R)-麦角甾烷-5, 7, 22-三烯-3β醇(7), β-谷甾醇(8), 麦角甾4, 6, 8(14)22-四烯-3-酮(9), (22E-24R)-麦角甾-7, 22-二烯-3β, 5a, 6β三醇(10), 胡萝卜苷(11), 棕榈酸(12), D-阿洛醇(13)和桑黄素D(14)。结论 化合物1~14均为首次从该菌中分离得到。
OBJECTIVE To determine the chemical constituents of Phellinus robustus. METHODS Some chromatographic METHODS were used to isolate the chemical constituents of this mushroom. Their structures were elucidated on the basis of spectral data. RESULTS Fourteen compounds were identified as:protocatechuic aldehyde(1), protocatechuic acid(2), coumarin(3), scopolin(4), (22E, 24R)-ergosta-7, 22-diene-3β-ol(5), 5α, 8α-epidioxy-22E-ergosta-6, 22-dien-3β-ol(6), 3β-hydroxy-(22E, 24R)-ergosta-5, 7, 22-triene(7), β-sitosterol(8), ergosta-4, 6, 8(14), 22-tetraen-3-one(9), (22E, 24R)-ergosta-7, 22-dien-3β, 5α, 6β-triol(10), daucosterin(11), palmitic acid(12), D-allitol(13) and phelligridin D(14). CONCLUSION All the fourteen compounds are isolated from Phellinus robustus for the first time.