中文摘要：

利用PCR技术从水稻（Oryza sativa）秀水11品种中克隆了转录因子WRKY19编码区5＇上游大小为1 404bp的调控序列,命名为OsW19p,将它和长度为105、378、977、1 106、1 205和1 306bp的5＇端缺失体分别与gus基因融合,构建植物表达载体.用根癌农杆菌介导法将所有载体转化水稻,GUS组织化学分析和荧光测定结果表明：（1）培养基中的2,4-D强烈抑制gus基因在愈伤组织阶段的表达;（2）gus基因在转基因植株的根、茎、叶和花中均有表达,在花粉和成熟种子中无表达,在种子萌发时胚有表达,在苗期种子根和不定根及它们的侧根均有表达但根尖无表达,成熟期根部只有侧根有表达;（3）除p105以外OsW19p（p1404）和其它5个不同长度的缺失体均可驱动gus基因在转基因苗叶片中的表达,p1205活性最高,p977和p1306的活性减弱,由此推测-1404～-1306 bp和-1205～-977 bp间包含正调控元件,-1306～-1205 bp和-977～-378 bp间包含负调控元件.

英文摘要：

A 1404 bp promoter fragment ofrice（Oryza sativa） transcription factor WRKY19 gene was amplified by PCR from rice Xiushuil 1 and named as OsW19p. The full length ofOsW19p（p1404） and its 5＇ end deletions with a length of 105, 378, 9 77, 1 106, 1 205 and 1 306 bp were fused with gus gene, respectively. All of the constructedvectors were transformed into rice calli by Agrobactetiurn-mediated method. The results of histochemical GUS staining and fluorometric measurements showed： （1） Expression of gus gene was intensively inhibited by 2,4-D in the culture medium at callus stage; （2） Expression ofgus gene was observed in roots, stems, leaves, flowers, and the embryos of germinating seeds. GUS staining were observed in advcntitious primary and lateral roots of rice seedlings, however, only lateral roots were stained at maturation phase; （3） GUS activity of all of deletions except p 105 were detected in leaves of transformed plants, the level ofp1205 was the highest, while expression was reduced for p977 and p1306. It suggests that some regulatory elements exist in -1404--1306 bp and -1205--977 bp, and fragments of -1306--1205 bp and -977--378 bp may contain negative elements.