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中文摘要：
以黔南60个野生茶树种质资源为材料,使用15对引物,通过SSR技术进行了DNA指纹数据库的构建。结果显示,所采用的15对引物共扩增出147个等位基因,有较好的多态性。位点的期望杂合度和多态性信息含量的变化范围分别为0.128～0.939和0.124～0.927,平均值分别为0.602和0.572。综合各项指标筛选出6对引物QNSSR01、QNSSR02、QNSSR04、QNSSR06、QNSSR18、QNSSR23上的23个等位基因用于黔南茶树种质资源DNA指纹图谱构建,60个茶树种质资源的SSR指纹图谱互不相同,可以作为各材料特定的图谱。研究结果为黔南茶树种质资源保护和品种创新利用奠定了基础。
英文摘要：
Sixty tea germplasm from Qiannan prefecture were collected to construct DNA fingerprint database by SSR technique. The results showed that 147 alleles were detected with considerable polymorphism by 15 pairs of primers employed in the experimental design. The ranges of expected heterozygosities and polymorphism information content at 15 SSR loci of 60 cultivars were 0. 128-0. 939 and 0. 124-0. 927 respectively ,with an average of 0. 602 and 0. 572 respectively. And a total of selected 23 alleles from 6 primers （ QNSSR01, QNSSR02, QNSSR04, QNSSR06 ,QNSSR18 ,and QNSSR23 ）were used for the DNA fingerprint construction. Sixty tea accessions with different SSR fingerprints of each other can serve as the cultivars-specific patterns and as an important basis for cuhivars identification.
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