中文摘要：

目的探讨亚硒酸钠诱导结直肠癌SW480细胞凋亡的信号通路。方法将SW480细胞分为三组,分别进行亚硒酸钠、Mn TMPy P以及两者联用处理;用DCFH-DA荧光探针法检测细胞内活性氧;Western blot方法检测细胞信号通路JNK和ATF-2的磷酸化,以及c-Myc、c-Jun、c-Fos、cyclin D1、cyclin A2、Bcl-2等相关蛋白表达;流式细胞术检测SW480细胞凋亡率。结果亚硒酸钠可使SW480细胞产生活性氧;抑制JNK和ATF-2的磷酸化（P〈0.05）,下调c-Myc、c-Jun、c-Fos、cyclin D1、cyclin A2、Bcl-2的表达诱导SW480细胞凋亡（P〈0.05）;用活性氧抑制剂Mn TMPy P可减轻亚硒酸钠所致的上述变化（P〈0.05）。结论亚硒酸钠可通过产生活性氧,抑制JNK和ATF-2的磷酸化,下调c-Myc、c-Jun、c-Fos、cyclin D1、cyclin A2、Bcl-2的表达,促进SW480细胞的凋亡。

英文摘要：

Objective To investigate the signaling pathway involved in the colorectal cancer SW480 cell apoptosis induced by selenite. Methods SW480 cells were divided into three groups,respectively treated with selenite,Mn TMPy P and their combinations. Monitoring the ROS content in SW480 cells by DCFH-DA fluorescence probes. The phosphorylation of JNK and ATF-2,and the expression of c-Myc,c-Jun,c-Fos,cyclin D1,cyclin A2,Bcl-2 and other related proteins were detected by Western blot. Cell apoptosis rate was detected by flow cytometry. Results Selenite can induce SW480 cells to produce ROS. Selenite can induce SW480 cell apoptosis by downregulating the phosphorylation of JNK and ATF-2,and the expression of c-Myc,c-Jun,c-Fos,cyclin D1,cyclin A2,Bcl-2（ P〈 0. 05）. The ROS inhibitor Mn TMPy P can reduce the effects caused by selenite（ P〈 0. 05）. Conclusions Selenite can induce ROS generation,inhibit JNK and ATF-2 phosphorylation and the expression of c-Myc,c-Jun,c-Fos,cyclin D1,cyclin A2 and Bcl-2,thus promote apoptosis in SW480 cells.