中文摘要：

目的研究新基因gasdermin3在小鼠同步化毛囊周期中表达的规律及意义。方法通过松蜡合剂脱去小鼠背部毛囊静止期毛发,诱导脱毛区域毛囊周期同步化,取脱毛诱导后5、9、18、25 d等4个时相点的材料作RT-PCR、Western blot以及免疫组织化学（IHC）检测。结果在所选择的4个时相点都检测到了gasdermin3的表达,RT-PCR与WB检测结果一致,在生长期（5、9 d）呈现表达递增的规律,退化期（18 d）表达降低,静止期（25 d）表达维持在很低的水平。IHC检测结果显示：gasdermin3蛋白表达定位在细胞核中;在表皮中持续表达,与毛囊周期无关;生长期gasdermin3表达在外根鞘、毛母质、内根鞘和毛干的细胞中;退化期gasdermin3表达在残留的内根鞘和外根鞘细胞中;静止期gasdermin3几乎没有表达。结论 gasdermin3在毛囊各个时期都有表达,其中在生长期晚期表达最高。gasdermin3蛋白的表达定位在细胞核中。gasdermin3可能作为转录因子,调控毛囊及表皮角质形成细胞的增殖、分化和凋亡。

英文摘要：

Objective To study the expression pattern of gasdermin3 gene in synchronized mouse hair cycle.Methods A beeswax/rosin mixture was employed to remove all hair on the dorsal skin of twelve eight-week-old C57BL/6 female mice and to induce hair cycle synchronization.The mice were randomly divided into four groups,each with three mice.The skin samples of time points at 5,9,18 and 25 d after depilation were collected and subjected to RT-PCR,Western blotting,and immunohistochemistry（IHC） testing.Results gasdermin3 expression was detected in the selected four time points.The results of RT-PCR and WB were consistent,indicating that the gene expression increased gradually in anagen（5 and 9 d）,decreased in catagen（18 d）,and stayed at a low level in telogen（25 d）.The IHC results demonstrated that gasdermin3 expression was localized in cell nuclei,and exhibited sustained expression in epidermis,independent of the hair cycle.gasdermin3 was expressed in the outer root sheath cells,hair matrix cells,inner root sheath cells,and hair shaft cells during anagen,as well as the residual inner root sheath cells and outer root sheath cells during catagen,but was rarely expressed during telogen.Conclusion gasdermin3 expression exists in all phases of hair cycle,showing the highest expression level in late anagen,and is localized in cell nuclei.Acting as a possible transcription factor,gasdermin3 regulates the proliferation,differentiation and apoptosis of hair follicles and epidermal keratinocytes.