研究了12株(K9、D17、C1、C12、D11、D14、C2、D9、K6、C21、D1和D7)分离自肉鸡肠道的乳酸菌的产乳酸能力及其中3株产酸能力强的菌株的耐受特性。12株乳酸菌产乳酸结果表明:12h内,K6产乳酸速度最快,其次为K9和C1,24h时,D17乳酸浓度最高,48h时C1终乳酸浓度最高。K9、D17和C1的耐受试验结果表明:C1菌株耐酸能力最强,pH2时,C1菌株培养3h后还能检测到活菌,D17和K9菌株培养1h后就已经检测不到活菌。在胆盐浓度0.08%-0.40%范围内,C1、D17和K9均有一定的耐受能力,随着胆盐浓度的升高,C1、D17和K9的存活数呈现缓慢的下降趋势。3株菌中D17耐热能力最强,经80%处理后仍有10^4.9/mL存活数,而K9和C1已检测不到活菌;C1对热最敏感,65℃处理后存活数由10^8/mL降为10^3/mL。
Lactic acid production of twelve strains of LAB isolated from broiler intestine and tolerance property of three strains were investigated. The results of lactic acid production showed that among all strains K6 exhibited the most rapid production during the first twelve hours, the seconds were K9 and C1 ; D17 exhibited the highest production of lactic acid by twenty-four hours, C1 exhibited the highest production of lactic acid by forty-eight hours. The pH values in three strains of K9, D17 and C1 culture showed the fast decline during the first twelve hours, with the final values significantly lower than those of other strains cultures. The results of tolerance property showed that the survival counts of Clcould be detected when pH value was at 2 after three hours, but the survival counts of D17 and K9 could not be detected after one hour. When pH value was at 2.5 after three hours , the survival counts of C1 declined from 10^8.2/mL to 10^4.8/mL, K9 from 10^8.2/mL to 10^4.6/mL, the survival counts of D17 could not be detected. 0. 08% bile had few effects on the survival counts of three strains; when incubated in the medium with 0. 40% bile, the survival counts of C1 declined from 10^8.4/mL to 10^6.5/mL, D17 from 10^10.3/mL to 10^7.5/mL, and K9 from 10^9.8/mL to 10^7.7/mL When the group treated with 37℃ for 20 minutes was served as the control, the survival counts of C1 and K9 was not detected when treated with 80℃, but the survival counts of D17 were 10^4.9/mL, when treatment with 65℃ the survival counts of C1 and K9 decreased significantly.