中文摘要：

目的检测人IL-6基因启动子能否在小鼠抗原递呈细胞内发挥启动活性及其是否具有可调控性。方法构建含不同cis元件IL-6启动子片段的荧光素酶报告基因的质粒，将其与对照质粒共转染小鼠树突状细胞和巨噬细胞株RAW264．7，在不同剂量LPS刺激条件下，检测荧光素酶的表达量。结果在3个ds元件中，NF-κB结合位点对于维持IL-6启动子的活性最为重要，LPS可诱导IL-6启动子在抗原递呈细胞内发挥活性，且在一定范围内随LPS的剂量而上升。结论IL-6启动子可作为一种可诱导型启动子在树突状细胞内发挥活性，因而有望用于基因治疗或基因功能研究。

英文摘要：

Objective To detect whether human IL-6 promoter can drive reporter gene expression and can be induced by some stimulus in antigen presenting cells. Methods Luciferase reporter vectors which contain variable cis elements of IL-6 promoter have been constructed and used to detect the promoter activity in mouse dendritic cells and macrophage cells RAW264.7 when stimulated by variable concentration of LPS. Results NF-κB binding site plays the most important role in controlling IL-6 transcription in antigen presenting cells. IL-6 promoter activity increases with the concentration of LPS in some degree. Conclusion IL-6 promoter can be used as an inducible promoter in researches of gene therapy and gene function.