中文摘要：

目的：观察水通道蛋白1（AQP1）在重症急性胰腺炎（SAP）大鼠肺组织中的表达,从新的角度探讨肺损伤（ALI）发生的机制。并观察清胰汤对SAP大鼠肺脏AQP1表达的影响。方法：Wistar大鼠制备SAP模型,随机分为4组：假手术对照组（SHAM组）、SAP模型组（SAP组）、SAP＋清胰汤治疗组（QYD组）、SAP＋地塞米松治疗组（DEX组）。各组又分为6、12、24 h组,分别于造模后6、12、24 h处死,取肺组织及动静脉血。检测血清淀粉酶水平（AMY）,测定动脉PaO_2、Pa CO2。取肺脏计算肺湿干重比值（W/D）、大体及镜下观察肺脏病理学改变,并用免疫组化和RT-PCR方法测定肺脏水通道蛋白1蛋白及m RNA的表达情况。结果：与SHAM组相比,SAP组AMY、Pa CO2、W/D值明显升高（P〈0.01）,PaO_2值明显下降,AQP1 m RNA和AQP 1蛋白表达明显下降（P〈0.01）。与SAP组相比,QYD治疗组AMY、Pa CO2、W/D值明显下降（P〈0.01）,PaO_2值明显升高（P〈0.01）,AQP1 m RNA和AQP 1蛋白表达明显升高（P〈0.01）。与SAP组相比,DEX治疗组AQP1 m RNA和AQP 1蛋白表达明显升高（P〈0.01）。DEX治疗组和QYD治疗组之间AQP1 m RNA和AQP 1蛋白表达无显著差异（P〉0.05）。结论：水通道蛋白1表达与急性胰腺炎损伤关系密切,清胰汤可上调其表达而减轻肺水肿。

英文摘要：

Objective： To observe the expression and function of Aquaporinl （ AQP1 ）in acute lung injury （ ALI） in rats induced by severe acute pancreatitis （ SAP ）, and to investigate the molecular mechanism of injury of lung in SAP. Methods ： Adult Wistar rats were randomly divided into 4 groups ： sham operation （ SHAM ） group, SAP group, dexamethasone （ DEX,2 rng/kg via caudal vein ） group and Qingyi Decoction （ QYD, 10 mL/kg ） group by intragastrie administration. Sodium deoxyeholate was （ 15 g/L ） injected inversely into the biliopancreatic duct of rats to make the model of SAP-induced ALI. Ten rats of each group （n=10）were killed 6, 12,24 h respectively after SAP model were established, and blood samples and lung tissues were collected. Serum amylase, partial pressure of oxygen and carbon dioxide, lung wet-to-dry weight ratio （ W/D ） and pulmonary homogenate were measured to evaluate the degrees of pancreatic. Reverse transeription-polymerase chain reaction （ RT-PCR ） was used to quantify AQP1 mRNA changes and immunohistoehemistry was used to detect AQP1 protein expression in lung tissues. Results： Compared with SHAM group, the levels of serum amylase, the value of W/D, the pathological ehangs of lung tissues and the partial pressure of carbon dioxide were significantly increased in SAP groups （P〈0.01）. While the partial pressure of oxygen, the expression of AQP1 mRNA and AQP 1 protein were significantly decreased （ P〈0.01 ）. After treatment with Qingyf Decoction, the value of W/D, the pathological decreased. Changs of lung tissues and the partial pressure of carbon dioxide were significantly decreased. And the the partial pressure of oxygen, the expression of AQPI mRNA and AQP1 protein were significantly increased. After treatment with dexamethasone the expression of AQP1 mRNA and AQP1 protein were also significantly increased. But there was no significantly difference between dexamethasone and Qingyi Decoction group （ P〉0.05 ）. Conclusion ： AQP1 may