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中文摘要：
目的：了解缺氧条件下体外培养人视网膜色素上皮（retinal pigment epithelium，RPE）细胞的凋亡情况。方法：将培养的人RPE细胞置于含10mL/LO2、50mL/LCO2和940mL/LN2的培养箱内建立缺氧模型。于缺氧后1，3，6，12，24h，利用扫描电镜、透射电镜、脱氧核糖核苷酸末端转移酶介导的缺口末端标记法（terminal-deoxynucle-otidyl transferase mediated nick end labeling，TUNEL）技术和流式细胞仪测定RPE细胞凋亡水平。结果：常氧状态下RPE细胞生长良好，几乎没有凋亡（TUNEL法测凋亡指数为1.2）。缺氧条件下，RPE细胞发生了不同程度的凋亡，缺氧后3h凋亡水平达峰值（凋亡指数为34.43）。结论：缺氧可导致体外培养的人RPE细胞凋亡，提示RPE凋亡可能参与了某些缺血缺氧性眼病的发生。
英文摘要：
AIM：To evaluate the effect of hypoxia on apoptosis of cultured human retinal pigment epithelial（RPE）cells.METHODS：In the hypoxia model,human REP cells were cultured in a special incubator containing volume fraction of 10mL/L O2,50mL/L CO2 and 940mL/L N2 for 0 hour,1 hour,3,6,12 and 24 hours.The level of apoptosis was individually measured with scanning and transmis-sion electron microscopy,terminal-deoxynucleotidyl transferase mediated nick end labeling（TUNEL）and flow cytometry.RESULTS：RPE cells grew well under normoxic conditions.The apoptosis was significantly activated and peaked at 3 hours in the cultured RPE cells under hypoxia.CONCLUSION：Hypoxia can induce apoptosis in the cultured RPE cells,suggesting that RPE apoptosis might play a role in ischemic ocular diseases.
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