中文摘要：

目的制备载介孔二氧化硅纳米囊脂质体（MSN-LIPO）,评价MSN-LIPO增强高强度聚焦超声（HIFU）对体外培养的肿瘤细胞的杀伤力。材料与方法采用薄膜水化法制备MSN-LIPO,用纳米粒度、电位分析仪和透射电子显微镜检测MSN-LIPO的基本表征;用CCK-8试验评估脂质浓度分别为0、0.1、0.2、0.5、1.0 mg/ml MSN-LIPO对U87 MG细胞的毒性;将FITC标记的MSN-LIPO与U87 MG细胞分别孵育0 min、15 min、30 min、1 h、2 h,使用共聚焦激光显微镜验证U87 MG细胞对不同分组MSN-LIPO的摄取;分别设立对照组、MSN-LIPO组、HIFU组和HIFU＋MSN-LIPO组,通过流式细胞仪评价不同分组对U87 MG细胞的杀伤作用。结果制备的MSN-LIPO粒径大小约为（128.60±2.11）nm,Zeta电位约为（-22.90±0.77）m V;不同浓度组MSN-LIPO对U87 MG细胞无显著毒性作用,组间差异无统计学意义（F=1.56,P〉0.05）;U87 MG恶性胶质瘤细胞能够成功摄取MSNLIPO,不同时间分组组间差异有统计学意义（F=675.81,P〈0.05）;不同治疗分组对U87 MG细胞具有不同程度的杀伤作用,组间差异有统计学意义（F=713.52,P〈0.05）。结论本研究制备的MSN-LIPO具有良好的生物相容性,可以协同增强HIFU对肿瘤细胞的杀伤力,具有潜在的临床应用价值。

英文摘要：

Purpose To prepare mesoporous silica nanocapsules loaded liposomes（MSNLIPO）, and to evaluate its synergistic effect of enhancing the therapeutic efficacy of high intensity focused ultrasound（HIFU） on tumor cells. Materials and Methods Thin film hydration method was used to prepare MSN-LIPO. The basic property of MSN-LIPO was characterized by Malvern Zetasizer Nano ZS and transmission electron microscopy. Cytotoxicity experiment was conducted to test the biocompatibility of MSN-LIPO with the concentrations of 0, 0.1, 0.2, 0.5 and 1.0 mg/ml. Confocal laser scanning microscopy was adopted to verify the uptake of MSN-LIPO marked by FITC by U87 MG tumor cells with the incubation time of 0 min, 15 min, 30 min, 1 h, and 2 h. Accordingly, the subjects were divided into control group, MSN-LIPO group, HIFU group and HIFU＋MSN-LIPO group. Flow cytometry was employed to evaluate the therapeutic efficacy of different treatment groups on tumor cells. Results The particle size and Zeta potential of MSNLIPO was（128.60±2.11） nm and（- 22.90±0.77） m V, respectively. Different concentrations of MSN-LIPO showed good biocompatibility to tumor cells; the differences between the groups had no statistical significance（F=1.56, P〉0.05）. The U87 MG tumor cells could uptake MSN-LIPO successfully; the groups with different time dependence showed statistical significance（F=675.81, P〈0.05）. Those groups with different treatment had different therapeutic efficacy on tumor cells; the differences presented statistical significance（F=713.52, P0.05）. Conclusion MSN-LIPO prepared in this study shows good biocompatibility and could synergistically enhance the therapeutic efficacy of HIFU on tumor cells, which has potential value in clinical application.