通过遮阴,使黄檗(Phellodendron amurense)幼苗生长在相对光强分别为100%、75%、50%和25%的环境下处理1130d,探讨光照强度对黄檗幼苗生长和抗氧化能力的影响。结果表明,黄檗幼苗在75%的光强下生长最好。随着光强进一步减弱,幼苗的鲜质量减少、根冠比增大、叶绿素含量增加而叶绿素a/b减小。随着环境光强的增加,黄檗幼苗的丙二醛(MDA)含量增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)的活性增强(相对光强75%的幼苗偶有例外)。强光虽然加剧了膜脂过氧化作用,但幼苗的抗氧化能力也有一定程度的提高。强光不利于黄檗幼苗期的生长发育。对于人工栽培的黄檗,在幼苗期应适当遮阴(试验的相对光强为75%),则更有利于其生长。
An experiment was conducted to study the effects of light intensity on seedling growth and anti-oxidative activities of amur corktree (Phellodendron amurense) under light intensitics of 100%, 75%, 50% and 25% of sunlight. Result showed that amur corktree seedlings had the best growth under 75% of sunlight. The fresh weight of amur corktree seedlings decreased, root/shoot ratio and chlorophyll content increased with the decrease of light intensity, while chlorophyll a/b dedined. Malondialdehyde (MDA) content, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) enhanced with the increase of light intensity except for some situations under 75% of sunlight. Antl-oxidative activity of seedlings increased although strong light strengthened membrane lipid peroxidation. Strong light is unfavourable for seedling development, and artificial culture benefits the growth of P. amurense seedlings under proper shading conditions (relative light intensity of 75% ).