中文摘要：

目的探讨五价砷酸钠暴露对Chang肝细胞核转录因子-E2类相关因子2（Nrf2）及其调控的下游抗氧化酶NAD（P）H：醌氧化还原酶1（NQO1）和血红素单加氧酶-1（HO-1）的mRNA及其蛋白表达的影响。方法 0.5、1.0、2.0mmol/L的砷酸钠（Na2HAsO4）染毒Chang肝细胞6 h后,采用western blot法检测细胞内Nrf2、NQO1和HO-1的蛋白表达情况;采用抗氧化反应元件（ARE）-荧光素酶活性检测Nrf2的转录活性情况;采用Real-time PCR检测细胞NQO1和HO-1的mRNA表达情况。结果 0.5、1.0、2.0 mmol/L的Na2HAsO4染毒6 h均能够显著诱导Chang细胞的Nrf2蛋白表达（P〈0.05）,ARE荧光素酶活性水平也随染毒剂量的增加而诱导,并且具有剂量-效应关系;随着染毒剂量的升高,HO-1和NQO1的mRNA及蛋白水平逐渐升高,与对照组相比具有统计学差异,并且具有剂量-效应关系（P〈0.01）。结论砷酸钠暴露能够诱导人类Chang肝细胞的Nrf2及其调控的下游抗氧化酶NQO1和HO-1的mRNA及其蛋白的表达。

英文摘要：

Objective To observe the effects of sodium arsenate on the mRNA and protein expression of transcription factor Nrf2 and its regulation of downstream antioxidant enzymes NAD（P）H,NQO1 and HO-1in Chang liver cells.Methods Chang liver cells were exposed to sodium arsenate（Na2HAsO4） at doses of 0.5,1 and 2 mmol / L for 6 hours respectively;Western blot assay was used to detect the protein expression of Nrf2,NQO1 and HO-1;ARE-luciferase activity was used to detect Nrf2 transcriptional activity;Real-time PCR assay was used to detect the mRAN of NQO1 and HO-1.Results Chang liver cells were exposed to Na2HAsO4（0.5,1 and 2 mmol/L） could significantly induce the protein expression of Nrf2（P0.05）;With the increase of exposure dose,the ARE luciferase activity level was induced in a dose-effect manner;mRNA and protein levels of HO-1 and NQO1 gradually increased with increasing does,there was a significant difference compared with the control group,and existed dose-effect relationship（P0.01）.Conclusion Sodium arsenate exposure could induce Nrf2 and the mRNA and protein expression of its regulation of downstream antioxidant enzymes NQO1 and HO-1 in the human Chang liver cells.