中文摘要：

目的研究脊髓Sirt1在大鼠化疗后神经病理性痛中的作用。方法造模分组：将健康成年雄性s-D大鼠18只随机分为3组：紫杉醇组（P组）、溶媒组（V组）、正常组（N组），每组6只。检测（-1、5、7、14天）各组大鼠机械缩足反射阈值（MWT）及脊髓中Sirt1、核因子-κB（NF-κB）和肿瘤坏死因子-α（TNF-α）mR-NA的含量。治疗分组：健康成年雄性s-D大鼠30只随机分为5组：紫杉醇组（P组）、紫杉醇＋DMSO组（P＋D组）、紫杉醇＋白藜芦醇组（P＋R组）、溶媒＋DMSO组（V＋D组）、溶媒＋白藜芦醇组（V＋R组），每组6只。测定各组大鼠（-1、7、8、9、10天）MWT，并在第10天检测各组大鼠脊髓中Sirt1、NF-κB和TNF-αmRNA的含量。结果与造模前1天相比，造模后5、7和14天紫杉醇组大鼠MWT明显降低。脊髓中Sirt1mRNA含量降低（P〈0．05）、NF-κB和TNF-α mRNA的含量增加（P〈0．01）。与紫杉醇＋DMSO组相比，第7、8、9、10天紫杉醇＋白藜芦醇组大鼠的MWT明显增加。第10天脊髓中Sirt1mRNA含量明显增加（P〈0．05），NF-κB和TNF-αmRNA的含量变化无统计学差异（P〉0．05）。结论激动脊髓中Sirt1信号可以减轻大鼠化疗药物引起的神经病理性痛。

英文摘要：

Objective To investigate the effects of siauin 1 （ Sirt1 ） on the neuropathic pain induced by paclitaxel. Methods A rat model of paelitaxel - induced pain was established through intraperitoneal injection of 2 mg/kg paclitaxel on Days 0, 2, 4 and 6. A total of 18 rats were randomly divided into the following groups （n = 6） ： a paclitaxel group （P） , a vehicle group （V） , and a naive group （N）. The mechanical withdrawal threshold （MWT） was evaluated. The levels of spinal nuclear factor - κB （ NF - κB ）, spinal Sial and tumor necrosis factor - α （ TNF - α ） mRNA were measured on the day before modeling and on Days 5,7 and 14. For treatment, another 30 rats were randomly divided into five groups （ n = 6 ） ： a paclitaxel group （P） , a paclitaxel ＋ DMSO group （ P ＋ D）, a paclitaxel ＋ Res group （ P ＋ R）, a vehicle ＋ DMSO group （V ＋ D） , and a vehicle ＋ Res group （V ＋ R）. The MWT of rats were evaluated on the day before modeling and on Days 7, 8, 9 and 10, while the levels of spinal Sirt1, NF - κB and TNF -α mRNA were meas- ured on Day 10. Results The paclitaxel group presented remarkable decreases in MWT and Sial mRNA （ P 〈 0.05 ） but significant increases in the levels of spinal NF - κB and TNF -α mRNA on Days 5, 7 and 14 （P 〈0.01 ） , compared with those on the day before modeling. The Paclitaxel ＋ Res group produced obvious increases in MWT on Days 7, 8, 9 and 10 and Sial mRNA on Day 10, compared with the Paclitaxel ＋ DMSO group （P 〈 0.05）. However, no statistical difference was found as to the levels of NF - κB and TNF - α mRNA （ P 〉 0. 05 ）. Conclusions Activation of spinal Sial can attenuate the neuropathic pain induced by paclitaxel in rats.