中文摘要：

目的 研究血清IL-8及IL-18水平在骨肉瘤患者与健康体检者之间的差异及临床意义。方法 采用酶联免疫吸附法（ELISA）检测160例骨肉瘤患者及250例健康体检者的外周血血清IL-8及IL-18水平,比较两组间IL-8、IL-18水平的差异及它们之间的相关性,探讨血清IL-8及IL-18水平与骨肉瘤发病及进展的关系。结果 骨肉瘤患者组血清IL-8水平较健康对照组升高（P〈0.01）,血清IL-18水平较健康对照组降低（P〈0.01）。在骨肉瘤患者组中,未发生远行转移的患者其血清IL-8水平低于已发生转移患者（P〈0.05）,未发生远行转移的患者其血清IL-18水平高于已发生转移患者（P〈0.05）。AJCC分期中Ⅰ-Ⅱ期的低度恶性肿瘤相对于Ⅲ期同一骨断不连续的肿瘤、Ⅳ期伴有血源性肺转移以及远行转移肿瘤其血清IL-8水平均较低（P〈0.01）,Ⅲ期较Ⅳ期伴有血源性肺转移以及远行转移肿瘤其血清IL-8水平略高,但差异无统计学意义（P〉0.05）;Ⅰ-Ⅱ期患者较Ⅲ、Ⅳ期患者血清IL-18水平均较高（P〈0.01）,Ⅲ期较Ⅳ期伴有血源性肺转移以及远行转移肿瘤其血清IL-18水平较高（P〈0.05）。结论 血清IL-8水平升高、IL-18水平下降可能与骨肉瘤发生、进展、转移有关,其作用可能表现为促进骨肉瘤细胞的增生和转移,两者有可能成为判断骨肉瘤的进展和预后的指标。

英文摘要：

Objective To investigate the difference of serum levels of interleukin-8 （ IL-8） and interleukin-18 （ IL-18 ） between osteosarcoma patients and healthy controls and its clinical significance.Methods The peripheral blood serum levels of IL-8 and IL-18 in 160 patients with osteosarcoma and 250 healthy controls were detected by Enzyme-linked immunosorbent assay （ ELISA） method.The difference and relation serum levels of IL-8 and IL-18 in these two groups were compared, and the relationship between serum levels of IL-8 and IL-18 and the pathogenesis and progression of osteosarcoma were explored. Results Serum levels of IL-8 in the osteosarcoma group significantly increased（P〈0.01） ,but the levels of IL-18 obviously decreased while comparing with those of the control group（P〈0.01） .In osteosarcoma group, the serum levels of IL-8 in patients without metastasis were lower than patients with metastasis （ P〈 0.05） , while serum levels of IL-18 in patients without metastasis were highei,（ P 〈 0 .05） .With respect to the AJCC staging,the serum levels of IL-8 of I ~ II low-grade malignant tumor were lower than that of stage with a bone broken discontinuous tumor, stage IV associated with hematogenous lung me-tastasis and metastasis tumor（ P〈0.01） .Serum levels of IL-8 of stage IH were slightly higher than those of stage IV, but the difference was not statistically significant（ P〉0.05） .Serum levels of IL-8 of stage I ？II were higher than those of stage IHand IV （ P〈 0 .01） .Serum levels of IL-8 stage IH with hematogenous metastasis of lung and tumor metastasis were higher than those of stage IV （ P 〈 0 .05） .Conclusion Increase of serum levels of IL-8 and decrease of serum levels of IL-18 may involve in the occun,ence,development and metastasis of the osteosarcoma,which may promote cell proliferation and metastasis.Serum levels of IL-8 and IL-18 can be used as a biomarker for detennining progress and prognosis of osteosarcoma