中文摘要：

目的探讨二甲双胍对甲状腺乳头状癌IHH-4细胞增殖的影响及其机制。方法 MTT法分别检测不同浓度的二甲双胍（0、5、10mmol/L）和二甲双胍10mmol/L在不同时间点（0、24、48、72h）对乳头状癌IHH-4细胞增殖的影响。Western blot法检测不同浓度二甲双胍干预IHH-4细胞48h后单磷酸腺苷激活的蛋白激酶α亚基（AMPKα）及磷酸化AMPKα（p-AMPKα）蛋白表达。结果二甲双胍可呈浓度和时间依赖性抑制IHH-4细胞增殖（P〈0.01）。随着二甲双胍干预浓度的增加,p-AMPKα/AMPKα表达量增加（P〈0.01）。结论二甲双胍可通过激活AMPK通路抑制甲状腺乳头状癌IHH-4细胞的增殖活性。

英文摘要：

Objective To explore the effect and mechanism of metformin on the proliferation of papillary thyroid carcinoma IHH-4 cells. Methods MTT assay was used to measure the proliferation of IHH-4 cells treated by different concentrations of metformin 0, 5 and 10 mmol/L and metformin 10 mmol/L at different time points of 0,24,48 and 72 hours. Western bolt was performed to detect the expressions of AMPKa and phospborylated AMPKa（p-AMPKa） of IHH-4 cells treated by different concentrations of meformin at 48 hours. Results Metformin could significantly inhibit IHH-4 cell proliferation in the concentration- and time-dependent manners（P〈0. 01）. With increasing concentrations of metformin, the expression level of p-AMPKa/AMPKa was significantly increased （P〈0. 01 ）. Conclusion Metformin can inhibit the proliferation activity of papillary thyroid cancer IHH-4 cells via activating AMPK pathway.