中文摘要：

目的：建立HPLC同时测定姜黄中姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素含量的测定方法，并对贵州（野生、种植）和全国其他不同产地姜黄中的3种姜黄素进行含量测定，为姜黄的质量控制提供参考。方法：采用Hypersil ODS2柱（250mm×4．6mm，5μm），流动相为乙腈-0．5％醋酸水溶液（44：56），流速1．0mL min^-1，检测波长425nm，柱温35℃。结果：姜黄素、脱甲氧基姜黄素、双脱甲氧基姜黄素的线性范围分别为0．001～1．59μg（r=0．9998），0．001～1．49μg（r=0．9999），0．001～1．55μg（r=0．9999）；平均回收率（n=9）分别为98．2％，97．4％，98．0％。结论：不同产地的姜黄中姜黄素、脱甲氧基姜黄素和双脱甲氧基姜黄素的含量有较大差别。

英文摘要：

Objective： To establish an HPLC method for simultaneous determination of curcumin, demethoxycurcumin and bidemethoxycurcumin of Curcuma longa L. The components of 33 raw herbs of Curcuma longa L. from different places of China were investigated. Method：An Hypersil ODS2 column （250 mm × 4.6 mm,5 μm）, with acetonitrile-0. 5% acetic acid（44：56）as mobile phase. The flow rate was 1.0 mL ·min^-1 and column temperature was maintained at 35 ℃. The detection wavelength was set at 425 nm for acquiring chromatograms. Results：The calibration curves of curcumin,demethoxycurcumin and bidemethoxycurcumin were linear in the range of 0. 001 - 1.59 μg（ r = 0. 9998 ） ,0. 001 - 1.49 μg （r = 0. 9999 ） ,0. 001 - 1.55 μg（ r = 0. 9999 ）, respectively;The average recoveries（ n = 9） were 98.2% ,97.4% and 98.0%, respectively. Conclusion：The very important findings are that curcumin, demethoxycurcumin and bidemethoxycurcumin of Curcuma longa L. will vary greatly in different locations.