中文摘要：

目的初步探讨军团菌感染与Toll样受体4（Toll-like receptor,TLR4）基因（2244G→A）及（2299A→G）多态性的关系。方法采用聚合酶链反应（PCR）扩增肺部感染患者痰液及尿液中军团菌属特异性16SrRNA基因,酶免疫检测法（EIA）检测尿液中血清1型嗜肺军团菌抗原;采用聚合酶链-限制性片段长度多态性（PCR-RFLP）技术检测25例军团菌感染者（病例组）、44例其他非革兰氏阴性菌引起的肺部感染者（患者对照组）和47名健康体检者（健康对照组）的TLR4基因（2244G→A）及（2299A→G）位点的多态性。结果病例组、患者对照组、健康对照组TLR4（2244G→A）位点基因型和等位基因频率差异有统计学意义（χ2=17.234,P=0.002;χ2=15.332,P〈0.001）;经两两比较,TLR4（2244G→A）位点基因型和等位基因频率病例组和患者对照组均高于健康对照组（P〈0.05）,病例组与患者对照组比较差异无统计学意义（P〉0.05）;3组TLR4（2299A→G）位点基因型和等位基因频率差异无统计学意义（P〉0.05）。结论 TLR4基因（2244G→A）位点多态性与军团菌和其他非革兰氏阴性菌易感性可能有关。

英文摘要：

Objective To investigate the relationship between Toll-like receptor4（TLR4） gene（2244G→A） or（2299A→G） polymorphism and Legionella infection. Methods The 16Sr gene of Legionella in sputum samples was amplified with polymerase chain reaction（PCR） and the antigen of Legionella pneumophila serogroup 1 strain was detcted with enzyme immune assay（EIA） for pulmonary infection patients.Polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） was used to analyze the TLR4（2244G→A） and（2299A→G） gene polymorphism in 25 Legionella infected patients,44 non-gram-negative bacterial infected patients,and 47 healthy individuals. Results There were significant differences among the three groups in TLR4（2244G→A） polymorphism（χ2=17.234,P=0.002;χ2=15.332,P0.001）.There was a significant difference between healthy controls and other groups in TLR4（2244G→A） polymorphism（P0.05）.There was no significant difference between Legionella infected patients and case controls（P0.05）.There was no significant difference among the three groups in TLR4（2299A→G） polymorphism（P0.05）. Conclusion TLR4（2244G→A） polymorphism is associated with the susceptibility of Legionella and other non-gram-negative bacterial infection.