中文摘要：

目的明确白及非多糖部分的止血作用,初步了解其止血机制.方法白及95%乙醇提取物上D101大孔树脂,经水洗脱后,用80%乙醇洗脱得到不含多糖的80%乙醇组分（BS-80EE）;以小鼠全身肝素化的出血时间（BT）和凝血时间（CT）为指标考察BS-80EE的止血作用;以二磷酸腺苷（ADP）为诱导剂,采用比浊法观察BS-80EE对大鼠体内血小板聚集率的影响;通过考察BS-80EE对大鼠凝血酶时间（TT）、凝血酶原时间（PT）、活化部分凝血活酶时间（APTT）和纤维蛋白原含量（FIB）,以及血小板膜糖蛋白P-选择素（P-S）、凝血酶-抗凝血酶复合物（TAT）、纤溶酶原激活物抑制剂-1（PAI-1）、D-二聚体（D-D）的影响,分别从血小板和凝血纤溶系统角度了解BS-80EE的止血机制.结果BS-80EE可剂量依赖性地缩短全身肝素化小鼠的CT和BT（P〈0.01或P〈0.05）;明显促进ADP诱导的大鼠体内血小板聚集作用（P〈0.01）;明显缩短大鼠的TT值（P〈0.01或P〈0.05）;高剂量组可明显增加FIB含量（P〈0.05）;中、高剂量组可明显增加P-S、TAT、PAI-1的含量,减少D-D的生成（P〈0.01）.BS-80EE虽有缩短大鼠APTT和PT的趋势,但差异均无显著性.结论白及非多糖组分（BS-80EE）具有良好的止血功效,可通过促进血小板聚集和凝血而发挥止血作用.

英文摘要：

Aim To understand the hemostatic effect ofa non-polysaccharide fraction of Bletilh striatt （ BS -80EE ） and to clarify its mechanism of action. MetliodsThe non-polysaccharide fraction （ BS -80EE ） wasprepared by passing the 9 5 % ethanol extract of BletiHthrough a D 101 macroporous resin column elutedfirst with water and then with 8 0 % ethanol. Bleedingtime （ ADP ） and clotting time （ CT ） of heparinizedmice were employed as indicators for evaluating the hemostaticeffect of BS -80EE . The mechanism of actionwas investigated through observing the effect of B S -80E E on platelet aggregation induced by adenosinediphosphate （ ADP ） in rats with nephelometry and testingthe effect of B S -80E E on the thrombin time（TT ） ,prothrombin time （ PT ） ,activated partial thromboplastintime（APTT ） , fibrinogen （ FIB） ,P -selectin（ P-S ） ,thrombin-antithrombin complex （ TAT ） , D-dimer （ D-D ） and plasminogen activator inhibitor-1 （ PAI-1 ） .Results B S -80E E significantly shortened the CT andADP （ P 〈0. 01 or 0. 05） of heparin mice in a dose-dependentmanner; groups of all doses significantly reducedthe rat TT （ P 〈 0. 01 or 0. 05 ）, and the highdosegroupsignificantly increased theFIB content （ P 〈0.05）; the mid-dose group and high-dose groups ofB S -80E E significantly increased the contents of P-S ,TAT and PAI-1,while reduced the D-D production inrats （ P 〈 0. 01 ） ; although dose-dependent reductionsof APTT and PT were observed for each treatmentgroup,no significance was observed. Conclusion B S -80E E possess pronounced hemostatic effect by promotingplatelet aggregation and coagulation.