目的:以小球藻Chlorella zofingiensis为研究对象,以葡萄糖和木糖为混合碳源,优化混合碳源浓度提高异养培养虾青素产量。方法:利用JMP软件中完全析因设计法考查葡萄糖和木糖浓度对虾青素产量的影响,并采用SAS软件对实验数据进行回归分析。结果:优化条件下葡萄糖和木糖浓度分别为26.86g/L和16.92g/L。结论:优化后的虾青素产量达到8.65mg/L,比初始条件产量7.39mg/L提高了17%。
Objective: Optimization of the concentration of glucose and xylose was carried out to increase astaxanthin yield by ChloreUa zof- ingiensis heterotrophically. Method:Full Factorial Design by software JMP was used to investigate the effects of glucose and xylose on the production of astaxanthin,and the experimental data was analyzed using regression analysis method by software SAS. Result:The optimal concentrations of glucose and xylose were 26. 86 g/L and 16. 92 g/L Conclusion: Astaxanthin yield of C. zofingiensis could reach 8.65 mg/L under optimum concentration,which was 17% higher than that achieved under original concentration (7. 39 mg/L).