通过正相硅胶、大孔吸附树脂、MCI树脂、Sephadex LH-20、制备薄层色谱和反相HPLC等多种色谱分离方法相结合,从甘青大戟乙醇提取物中分离得到16个萜类化合物;借助波谱学分析方法鉴定它们的结构分别为:黑麦草内酯肉豆蔻酸酯(1)、24-甲烯基甘遂烷-8-烯-3β,11α二醇-7-酮(2)、黑麦草内酯(3)、3β-hydroxy-5α,6α-epoxy-7-megastigmene-9-one(4)、续随子醇A(5)、续随子醇D(6)、latilagascene F(7)、泽漆内酯A(8)、泽漆内酯B(9)、3-O-乙酰基泽漆内酯B(10)、泽漆内酯D(11)、泽漆内酯E(12)、(+)-11-乙酰氧基阿替烷-16-烯-3,14-二酮(13)、高根二醇(14)、熊果醇(15)和白桦脂醇(16)。以上化合物均为首次从甘青大戟中分离得到,其中1和2为新化合物。
From an ethanol extract of Euphorbia micractina roots, sixteen terpenoids were isolated by a combination of various chromatographic techniques, including column chromatography over macroporous resin, silica gel, and Sephadex LH-20 and reversed-phase HPLC. Their structures were elucidated by spectroscopic data analysis as loliolide myristate(1), 24-methylenetirucall-8-en-3β,11α-diol-7-one(2), loliolide(3), 3β-hydroxy- 5α,6α-epoxy-7-megastigmen-9-one(4), jolkinol A(5), jolkinol D(6), latilagascene F(7), helioscopinolide A(8), helioscopinolide B(9), 3-O-acetylhelioscopinolide B(10), helioscopinolide D(11), helioscopinolide E(12),(+)-11-acetoxyatis-16-en-3,14-dione(13), erythrodiol(14), uvaol(15) and betulin(16). All of the compounds were obtained from this plant for the first time, in which 1 and 2 are new compounds.