中文摘要：

探讨茉莉酸甲酯对人神经母细胞瘤细胞株BE（2）-C的生长抑制作用及其对凋亡抑制蛋白家族成员XIAP和survivin表达的影响。1-2 mmol·L^-1茉莉酸甲酯处理BE（2）-C细胞6-24 h后，MTT法检测瘤细胞生长活性，克隆形成实验检测细胞增殖能力，PI单染流式细胞术检测细胞周期，AO/EB荧光染色法、Hoechst 33258荧光染色法、Annexin V-FITC/PI双染流式细胞术检测细胞凋亡，半定量逆转录聚合酶链反应（RT-PCR）、实时荧光定量逆转录聚合酶链反应（real-time RT-PCR）检测细胞周期素D1（cyclin D1）、X连锁凋亡抑制蛋白（X-linked inhibitor ofapoptosis protein，XIAP）及survivin表达。结果表明，各浓度茉莉酸甲酯作用后，BE（2）-C细胞呈时间、浓度依赖性生长抑制，1，1.5和2 mmol·L^-1茉莉酸甲酯作用24 h后，细胞生长抑制率为20.6%-85.5%（P〈0.01），IC50为1.35 mmol·L^-1；S期细胞比率增高，部分瘤细胞发生典型的凋亡形态学改变，早期凋亡细胞（FITC^＋PI^-）分别占13.51%、17.32%和24.59%（均P〈0.01），细胞死亡率分别为29.36%、54.73%和75.52%（均P〈0.01）；XIAP和survivin表达分别下调18.5%-68.9%和22.4%-48.7%（均P〈0.05），而cyclin D1表达无显著变化（P〉0.05）。可见，茉莉酸甲酯能通过诱导细胞周期阻滞和凋亡抑制人神经母细胞瘤细胞株BE（2）-C的生长活性，下调XIAP和survivin基因表达可能是其作用机制之一。

英文摘要：

This study is to explore the inhibitory effect of methyl jasmonate on cell proliferation and expression of XIAP and survivin of human neuroblastoma cell line BE （2）-C. After cultivation of 1 - 2 mmol·L^-1jasmonates with BE （2）-C cells for 6 -24 h, the growth inhibiting rates of BE （2）-C cells were studied by MTT colorimetry. Cell proliferation was detected by colony formation assay. Cell cycle phases were assayed by propidium iodide staining flow cytometery. Cell apoptosis was inspected by acridine orange-ethidium bromide fluorescent staining, Hoechst 33258 fluorescent staining, and Annexin V-FITC and propidium iodide staining flow cytometry. Expressions of cyclin D1, XIAP and survivin were determined by RT-PCR and real-time RT-PCR. Methyl jasmonate inhibited the growth of BE （2）-C cells in a dose- and time-dependent manner. After addition of 1, 1.5 and 2 mmol·L^-1 of methyl jasmonate for 24 h, the inhibiting rates of cell growth reached 20.6% -85.5% （P〈0.01）, and the IC50 was 1.35 mmol·L^-1. The cell cycles were arrested at S phase. A part of cells presented the characteristic morphological changes of apoptosis. The early apoptotic rates were 13.51%, 17.32%, 24.59% （P〈0.01） and the cell death rates were 29.36%, 54.73%, 75.52% （P〈0.01）, respectively. The expression of XIAP and survivin mRNA were downregulated by 18.5% - 68.9% , 22.4% - 48.7% （P〈0.05）, respectively, without change in that of cyclin D1. The results indicated that methyl jasmonate could significantly inhibit the growth of BE （2）-C cells through inducing cell cycle arrest and apoptosis, downregulating the expression of XIAP and survivin might be one of its molecular mechanisms of action.