中文摘要：

利用PCR方法,从自身不合成γ-聚谷氨酸（γ-PGA）的Bacillussubtilis168菌的基因组DNA中扩增出γ-PGA合成基因ywsC,ywtA和ywtB,测序并对该基因编码区进行序列分析,比对结果表明,扩增的ywsC,ywtA和ywtB与文献报道的相似度为100%.将3个基因连接到pTrcHisA载体后转化至E.coliTOP10及E.coliBL21（DE3）宿主菌表达,结果宿主菌细胞均具备了γ-PGA生物合成能力,产量最高达到0.134g/L.

英文摘要：

The genes,ywsC,ywtA and ywtB,involving in biosynthesis of poly-γ-glutamic acid were amplified by PCR from the genome of Bacillus subtills 168.Sequence blast result showed that the sequence of target genes was 100% the same as that of B.subtilis 168.The genes were then inserted to the plasmid pTrcHisA and transformed into E.coli TOP10 and E.coli BL21（DE3） host cells.The result indicated that both the strains obtained the ability to biosynthesize poly-γ-glutamic acid,and the highest yield of γ-PGA reached 0.134 g/L.