欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
本试验建立和优化了重组蛋白rSEFA介导的间接ELISA方法以特异性检测肠炎沙门菌感染血清。确定其抗原最佳包被量为7．5p,g／mL，血清最适稀释度1：100，作用时间为60min；酶标二抗最适稀释度为1：4000，作用时间为60min；判定标准为OD值≥0．346。基于rSEFA介导的间接ELISA有较好的特异性，能特异性识别肠炎沙门菌和都柏林沙门菌感染血清，不能识别相近的鸡白痢沙门菌感染血清，此方法与菌体凝集反应同时检测临床血清样品100份，二者阳性率分别为67％和54％，结果符合率为80．6％。这一方法的建立对肠炎沙门菌特异性抗体检测有潜在的应用前景。
英文摘要：
An indirect ELISA assay was developed for detection of S.enteritidis using a recombinant fusion antigen rSEFA. This assay was optimized for antigen coating centration of 7.5μg/mL and a serum dilutin of 1：100,with a standard incubation time of 60 min. A reading of OD490≥0.346 was scored as positive. The recombinant antigen had no cross reaction with sera of other relative disease. The result also showed the indirect ELISA was highly sensitive ,specific and reproducible. The application of indirect ELISA would provide a simple and rapid method for monitoring serum antibodies against S.enteritidis infection.
同期刊论文项目
同项目期刊论文
Genetic Variation at the alpha-1-fucosyltransferase(FUT1) gene in Asia wild boar and Chinese and Wes
Genetic variation at the alpha-1-fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and W
Genetic Diversity, Genetic Distance and Phylogeny of Functional ApoVLDL-II and Lipoprotein Lipase Ge
Organ- and Host-Specific Clonal Groups of Staphylococcus aureus from Human Infections and Bovine Mas
期刊信息
位置：