中文摘要：

目的探讨不同周龄小鼠动脉粥样硬化（atherosclerosis，AS）模型中miR-23a与白介素-6（IL-6）、单核细胞趋化蛋白-1（MCP-1）和肿瘤坏死因子-仅（TNF-α）等血清炎症因子的相关性。方法选取12周龄、14周龄、16周龄、18周龄ApoE基因敲除小鼠（ApoE-／-小鼠），各6例，分别采集各组小鼠血清进行实时荧光定量PCR（RT—PCR）检测miR-23a的表达情况，用酶联免疫吸附法（ELISA）检测不同周龄小鼠血清IL-6、MCP-1和TNF—α炎症因子的表达水平。结果随着ApoE-／-小鼠周龄的增加，小鼠血清中miR-23a表达呈逐渐降低趋势，而小鼠血清中的IL-6、MCP-1、TNF—α的表达明显升高。各组问比较差异均有统计学意义（P〈0．05）。结论在ApoE-／-小鼠As形成过程中，随着miR-23a表达水平下降，负向控制IL-6、MCP-1和TNF-α在内的多种炎症因子的表达，增加主动脉组织内的炎症反应水平，加速AS的发生发展。

英文摘要：

Objective To investigate the correlation between miR-23a and interleukin-6 （IL- 6）, monocyte chemoattractant protein-1 （MCP-1） and tumor necrosis factor-α （TNF-α） and other inflammatory factors in atherosclerotic （AS） models of different age mice. Methods ApoE knockout mice （ApoE-/- mice） at 12 weeks old, 14 weeks old, 16 weeks old and 18 weeks old were selected in 6 cases, the serum of each group was collected and the expression of miR-23a was detected by real-time quantitative PCR （RT-PCR） , and the levels of IL-6, MCP- 1 and TNF-a in the serum of different age mice were detected by enzyme-linked immunosorbent assay （ELISA）. Results With the increase of the age of ApoE-/- mice, the expression of miR-23a in mice serum gradually decreased, while the expression of IL-6, MCP-1 and TNF-α in serum was increased with a statistical significance （P 〈 0. 05）. Conclusion In the process of AS formation of ApoE-/- mice, with the decrease of miR-23a expression level, negative con- trol of a variety of inflammatory factors, including IL-6, MCP-1 and TNF-α, increase the level of inflammatory reaction within the aorta, accelerate the occurrence and development of AS.