中文摘要：

目的：探讨有氧运动对自发性高血压大鼠脑动脉平滑肌RyR-BKCa耦联的影响。方法：12周龄雄性自发性高血压大鼠（Spontaneous hypertension rat,SHR）以及正常血压对照组（Wistar-Kyoto,WKY）,随机分为正常血压安静组（WKY-SED）,正常血压运动组（WKY-EX）,高血压安静组（SHR-SED）和高血压运动组（SHR-EX）;运动组进行8周中等强度跑台运动训练。8周后,取脑动脉,酶消化法急性分离脑动脉平滑肌细胞,分别采用穿孔膜片钳和单通道内面向外模式记录瞬时外向电流（STOC）以及BKCa通道门控特性;钙离子成像技术观察胞内钙离子浓度变化;免疫荧光成像观察平滑肌细胞BKCa通道α、β1亚基表达及分布情况。结果：1）经8周有氧运动,WKY和SHR运动组收缩压都显著低于各自安静组;2）穿孔膜片钳结果显示,SHR-SED组STOC幅值显著高于WKY-SED组,SHR-EX组STOC幅值显著低于SHR-SED组,而各组STOC频率无显著性改变;3）与WKY-SED组相比,SHR-SED组BKCa通道开放概率（Po）显著增加,SHR-EX组开放概率显著低于SHR-SED组,但WKYEX组开放概率却高于WKY-SED组;4）在他莫昔芬激活下,WKY-SED和SHR-SED组BKCa通道开放概率分别增加3.4倍和8.9倍,WKY-EX组开放概率显著高于WKY-SED组,但与SHR-SED组相比,运动显著抑制SHR-EX组他莫昔芬诱发的开放概率增加;5）STOC可被RyR激动剂咖啡因激活;咖啡因能够引起肌质网钙库释放,诱发全胞钙瞬变,各组钙释放幅值无显著性差异;6）BKCa通道α、β1亚基存在于平滑肌细胞质膜上,β1亚基在胞内也有散在分布,α亚基在各组表达无显著性差异,β1亚基在WKY-EX和SHR-SED组中表达均显著高于WKY-SED组,有氧运动显著降低了SHR-EX组β1亚基表达量。结论：高血压引起RyRBKCa功能耦联的增强是为了缓冲血管压力增高诱发的脑动脉收缩,而BKCa通道β1亚基功能上调是其重要因素之一;长期规律有氧运动能有效地抑制这种病理性的?

英文摘要：

Objective：The purpose of this study was to investigate the effects of aerobic exercise on the functional coupling of RyR-BKCachannel in cerebral arterial smooth muscle cells from spontaneously hypertensive rats.Methods：12-week-old male SHR and WKY rats were randomly assigned to sedentary groups（SHR-SED,WKY-SED）and exercise training groups（SHR-EX,WKY-EX）.Exercise groups were performed an 8-week moderate-intensity treadmill running.After 8weeks,cerebral arterial smooth muscle cells were enzymatically isolated.Spontaneous transient outward currents（STOC）and BKCasingle channel currents were measured using whole-cell and inside-out patch,respectively.Cytosolic Ca2＋response was acquired using Ca2＋image;Immunofluorescence was performed to study the expression of BKCachannelαandβ11 protein.Results：1）After 8 weeks of exercise,SBP in both WKY-EX and SHR-EX were significantly lower than that of their sedentary counterparts.2）The amplitude of STOC in SHR-SED was significantly higher than that in WKY-SED;After exercise,it was significantly lower in the SHR-EX than in SHR-SED;There was no significant difference of the frequency among four groups.3）The Po of BKCachannels from SHR-SED was larger than that of WKY-SED;The Po in SHR-EX became lower than in SHR-SED;in normotensive rats,the Po after exercise training（WKY-EX）was significantly higher than WKY-SED.4）Tamoxifen evoked a 3.4-fold and 8.9-fold increase in the Po of BKCachannels in WKY-SED and SHRSED patches,respectively;In WKY-EX,tamoxifen-evoked Po was significantly higher than in WKY-SED ones;However,in hypertensive rats,exercise training inhibited tamoxifen-evoked effects.5）The RyR agonist caffeine activated STOC effectively;Caffeine could lead to the release of SR Ca2＋store,inducing cytosolic Ca2＋transients,rapid application of caffeine evoked similar Ca2＋transients in four groups.6）BKCachannelαandβ11subunits were plasma membrane-localized in arterial myocytes,besides a large fraction ofβ11subunits were intracellular;The