大石鸡是我国特有种,分布区狭窄;石鸡为广布种;两者都是我国北方干旱、半干旱荒漠环境的指示鸟类。利用线粒体DNA细胞色素b基因标记分析了大石鸡106个、石鸡48个样品,获得大石鸡单倍型9个,石鸡单倍型13个;在大石鸡种群中发现8个个体呈现石鸡基因模式(Hap-1和Hap-2),但是在石鸡种群中没有发现大石鸡的基因模式,确认为石鸡和大石鸡之间的渐渗杂交;确认杂交带从六盘山脉向南到陇南地区的礼县一带,杂交带长度为400km,宽度为130km。结果表明目前渐渗杂交对大石鸡种群遗传多样性影响不大,在不长的进化时期内,对增加物种多样性是有好处的。建议进行长期野外监测,以便弄清发生渐渗杂交的野外生态学数据,为物种进化研究提供参考依据。
Przevalski partridge (Alectoris magna) is endemic in China with limited range. Chukar partridge (Alectoris chukar), however has broad distribution. Both of them are indicative birds of arid and semiarid environments in northern China. We sampled 106 Przevalski partridges and 48 Chukar partridges for Cytb analyzing in the use of polymerase chain reaction (PCR) and direct sequencing methods. 9 haplotypes of przevalski partridges and 13 haplotypes of chukar partridges were found; There were 8 Przevalski partridges from five populations having Chukar partridge Cytb genotype. However, there were no Chukar partridge with Przevalski partridge' s genotype. Thus, we can make it sure that the hybridization between two partridges is introgressive hybridization. The introgressive hy- bridization is about 130 km wide and 400 km long from the surrounding of the Liupan Mountains to Lixian County. Analysis of Cytb gene indicates that introgressive hybridization has no significant influence on genetic diversity of Przevalski partridge population. As a result, introgressive hybridization is good to crease diversity of partridges in a short evolutionary time. In order to collect relative detailed data, long term wild monitoring should be carried on, which could contribute to species evolution research.