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中文摘要：
目的：探讨紫杉醇对大鼠血管平滑肌细胞（VSMC）和血管内皮细胞（VEC）G1-S检查点的作用。方法：将大鼠VSMC与VEC进行原代培养，50nmol／L紫杉醇干预72h后进行免疫荧光染色和蛋白印迹法检测。结果：同VEC相比，紫杉醇干预的VSMC中Brdu表达明显增加（均P〈0．05）。紫杉醇干预的VSMC中cyclinD1、P27表达高于VEC，P21表达较低（均P〈0．05）。结论：紫杉醇干预的VSMC同VEC相比，在增殖率与细胞周期调节因子表达方面具有明显差异，紫杉醇对VSMCG1-S检查点有特异性调节作用。
英文摘要：
Objective： To explore the effects of paclitaxel on G1 -S checkpoint of vascular smooth muscle cell （VSMC） and vascular endothelial cell （VEC） of rats. Methods： VSMC and VEC of rats underwent primary culture and were intervented by 50 nmol/L paclitaxel for 72 hours and then evaluated by immunohistochemistry and western blotting. Results： Compared with VEC, the content of BrdU was increased in paclitaxel-treated VSMC （P 〈 0.05）, paclitaxel-treated VSMC could express more cyclin D1, P27 and fewer P21 （P 〈 0. 05 ）. Conclusions ： Paclitaxel-treated VSMC shows significant differences in the expressions of cell cycle regulators and proliferation rates as compared with those of VEC, thus suggests the specific G1 -S checkpoint regulation effect of paclitaxel on VSMC.
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