中文摘要：

目的：探讨8周耐力训练对骨骼肌谷胱甘肽抗氧化系统的影响,以及Nrf2基因缺失的作用。方法：C57BL/6J野生型小鼠、Nrf2基因敲除小鼠各20只,随机分为安静对照组和耐力训练组,每组10只。耐力训练组采取坡度为0°、速度为12 m/min的跑台训练,1 h/d,5 d/周,共8周。完成训练48 h后取小鼠腿部骨骼肌,Western blot测定骨骼肌Nrf2、GSH-Px、GR、GCLc和GCLm蛋白表达,酶还原法测定骨骼肌GSH、GSSG含量。结果：1）Nrf2敲除鼠与野生鼠相比,骨骼肌内GSH-Px（P〈0.05）、GR（P〈0.05）、GCLc（P〈0.01）蛋白表达,GSH（P〈0.01）、GSSG（P〈0.05）含量和GSH/GSSG比值（P〈0.05）非常显著性或显著性下降,而GCLm蛋白表达无明显差异。2）耐力训练组与安静对照组相比,野生训练鼠骨骼肌Nrf2、GSH-Px、GCLc、GCLm以及GSH/GSSG比值均呈上升趋势,GSSG含量减少;Nrf2敲除训练鼠的谷胱甘肽相关酶蛋白表达以及GSH/GSSG比值则表现下降趋势,GSSG含量上升,但并无显著性。结论：Nrf2的缺失导致耐力训练不但没有提高机体抗氧化能力,反而加剧机体抗氧化水平的下降。

英文摘要：

The study aimed at investigating the effect of eight-week endurance training on glutathione antioxidant system and the role of nuclear factor erythroid-2 related 2（Nrf2）.Methods：Twenty two-month-old C57 BL/6J wild-type（WT） mice and twenty Nrf2 knock-out（KO） mice were divided into control and endurance exercise groups（n=10）.Mice in exercise groups underwent 60 min/day treadmill running at speed of 12 m/min for 8weeks.Skeletal muscle was collected 48 hours after training.The protein expressions of Nrf2,GSH-Px,GR,GCLc and GCLm in skeletal muscle were analyzed by Western blot.The GSH and GSSG levels were tested by Enzyme reduction method.Results：1） Compared with WT mice,the expressions of GSH,GSSG,GSH/GSSG,GSH-Px,GR and GCLc of skeletal muscle in Nrf2 knock-out mice significantly decreased while GCLm had no difference.2） Compared with control group,the expressions of Nrf2,GSH-Px,GCLc and GCLm and GSH/GSSG value of WT mice in exercise group increased while GSSG decreased,the expressions of glutathione related enzymes and GSH/GSSG value of Nrf2 knock-out mice in exercise group decreased while GSSG increased,but no difference.Conclusion：The genetic depletion of Nrf2 doesn’t improve antioxidant capacity,and it decreases antioxidant level.