中文摘要：

采用超高效液相色谱-质谱联用（UPLC-MS/MS）方法研究了阿卡波糖对Ⅱ型糖尿病大鼠代谢轮廓的影响,分析了健康组、糖尿病模型组和糖尿病给予阿卡波糖组的大鼠尿样,采用主成分分析法（PCA）和偏最小二乘法-判别分析（PLS-DA）对数据进行分析.PCA得分图表明,健康组、糖尿病组和阿卡波糖组的代谢轮廓有显著差别,根据PLS-DA载荷图筛选,将对各组分离贡献大的化合物的串联质谱分析数据经HumanMetabolome Database（HMDB）和Mass Bank.jp等数据库检索,进行质谱信息匹配,鉴定出苯乙酰甘氨酸、肌酐及葡萄糖酸等8种内源性代谢物为潜在生物标记物.

英文摘要：

An ultra performance liquid chromatography-mass spectrometry（UPLC-MS） method was developed for the metahonomics study of effect of acarbose on type Ⅱdiabete rats. Urine from three groups of rats including control group, model group and diabete rats treated with acarbose group were analyzed, and the data were analyzed by the method of principal component analysis（PCA） and partial least squares-discriminant analysis （PLS-DA）. The PCA score plots showed that the three groups were significantly different between the metabolic profile. According to the results of PLS-DA, the MS/MS data of each compound which provide greater contribution to separation of each group was searched from the Human Metabolome Database （HMDB） and Mass Bank. jp databases. Eight kinds of endogenous metabolites were identified as potential biomarkers such as gluconic acid, phenylacetylglycine, creatinine, etc.