中文摘要：

【目的】证明纤维肉瘤病料中含有的ALV-J相关病毒能够诱发急性纤维肉瘤。【方法】将含ALV-J相关病毒的病料过滤液经不同部位接种1日龄817肉杂鸡；将该过滤液不同倍数稀释后接种1日龄SPF鸡和817肉杂鸡；将该过滤液接种细胞培养后的上清液接种1日龄817肉杂鸡，连续动态观测30d。【结果】颈部皮下、胸肌、腹腔3个部位攻毒鸡的肿瘤发生率分别为100％（20／20）、95．2％（20／21）、100％（20／20）。病料过滤液未经稀释接种817肉杂鸡和SPF鸡的肿瘤发生率均为100％（1o／10），10倍稀释后攻毒鸡的肿瘤发生率分别为80％（8／10）和100％（10／10），100倍稀释后攻毒鸡的肿瘤发生率分别为100％（10／10）和80％（8／10），1000倍稀释后攻毒鸡的肿瘤发生率分别为40％（4／10）和50％（5／10），更高倍数稀释后攻毒鸡没有肿瘤发生。接种病料的细胞培养上清也能使33．3％（2／6）的鸡只发生肉瘤。病理组织学检测显示：诱发的急性肿瘤系典型的纤维肉瘤。【结论】817肉杂鸡肉瘤病料中含有ALV-J相关的急性致肿瘤病毒，不论是病料过滤液本身还是其接种DF-1细胞培养后的上清液都能在不同品种鸡复制出急性纤维肉瘤，最快在接种后7d出现，且肿瘤发生的时间、动态和肿瘤发生率与接种量密切相关。

英文摘要：

[Objective] An experiment was carried out to prove that the field chicken fibrosarcoma mass contains acute fibrosarcoma-inducing ALV-J associated virus. [Method] One-day-old 817 hybrid chickens were inoculated through different routes with tumor suspension filtrate containing ALV-J associated virus. One-day-old 817 hybrid chickens and SPF Leghan chickens were inoculated subcutaneously with tumor suspension filtrate containing ALV-J associated virus in different doses. Continuous observation of tumor development was made in 30 days. [Result] In the groups of inoculations through the subcutaneous, in muscule and intra-abdominal, the tumor incidence was 100% （20/20）, 95.2% （20/21）, and 100% （20/20）, respectively. The tumor incidence of 817 hybrids and SPF chickens inoculated with undiluted tumor extract filtrate was 100% （10/10）. When birds were inoculated with tumor extracts in 1 ： 10 and 1 ： 100, tumor rates were still in the range of 80% （8/10） to 100% （10/10） for both chicken lines. When birds were inoculated with tumor extracts in 1 ： 1 000, tumor rates decreased to 40% （4/10） or 50% （5/10）. No tumor development was observed in 30 days when birds were inoculated with the tumor extract of 1 ： 10 000 dilution. However, there were 2/6 chickens developed tumor mass when inoculated with supematant from tumor extract-infected DF-1 cell culture. Histopathology indicated that the induced tumors were typical fibrosarcoma. [ Conclusion ] The fibrosarcoma tissues of 817 hybrid chickens of field case contained ALV-J associated acutely tumorigenic virus. Both tumor extract filtrate or extract-infected DF-1 cell culture supernatant could induce acute fibrosarcomas in chickens of different lines, and the tumor started to appear as early as 7 days after inoculation. Thedynamics of the fibrosarcoma development and tumor rates were closely related to doses of the tumor extracts in inoculation.