中文摘要：

瞄准：在人的直肠的癌症澄清导致 Wnt 的分泌 protein-1 (WISP-1 ) 的表示变化并且决定它是否与人的直肠的癌症的侵略和转移被相关。方法：直肠的癌症并且通过手术的 86 件配对的样品 resected 远正常直肠的织物分别地进癌症组和控制组镇定、分配。WISP-1 mRNA 被相对量的即时 RT-PCR 检测， WISP-1 蛋白质被免疫检验组织化学的染色。结果：在表示上的 WISP-1 基因在 65% 被发现(56/86 ) 主要直肠的癌症，乘在正常的水平的 2-30 匹配直肠的纸巾(P = 0.001 ) 。mRNA 表示水平与公爵的阶段，组织分化等级和淋巴节点地位被相关。WISP-1 蛋白质表示根据 mRNA 表示水平。免疫的积极的度在癌症组的组织化学的染色(1.40 +/- 0.35 ) 在控制组与那不同(1.04 +/- 0.08， P 【 0.001 ) 。而且，在高级 mRNA 癌症在癌症组织积极染色度(1.46 +/- 0.37， n = 56 ) 在低级 mRNA 比那高(1.28 +/- 0.28， n = 30， P = 0.018 ) 。结论：WISP-1 表示的异常层次可以在直肠的肿瘤发生起一个作用。WISP-1 可以在直肠的癌症被用作特定的临床诊断和预后标记。

英文摘要：

AIM： To clarify the expression change of Wnt-induced secreted protein-1 （WISP-l） in human rectal cancer and to determine whether it is correlated with invasion and metastasis of human rectal cancer. METHODS： Eighty-six paired samples of rectal cancer and surgically resected distant normal rectal tissue were collected and allocated into cancer group and control group respectively. WISP-1 mRNA was detected by relative quantitative real-time RT-PCR and WISP-1 protein was examined by immunohistochemical staining. RESULTS： WISP-1 gene overexpression was found in 65% （56/86） primary rectal cancers, 2-30 times that of the level in normal matched rectal tissues （P = 0.001）. The mRNA expression level was correlated with Duke＇s staging, histological differentiation grade and lymph node status. The WISP-1 protein expression was in accordance with mRNA expression level. The positive degree of immunohistochemical staining in the cancer group （1.40±0.35） was different from that in control group （1.04± 0.08, P 〈 0.001）. Moreover, in cancer group the positive staining degree in high-level mRNA cancers （1.46 ±0.37, n = 56） was higher than that in low-level mRNA （1.28 ± 0.28, n = 30, P = 0.018）. CONCLUSION： Aberrant levels of WISP-1 expression may play a role in rectal tumorigenesis. WISP-1 may be used as a specific clinical diagnosis and prognosis marker in rectal cancer.