中文摘要：

目的：研究丹参与丹皮配伍的化学成分。方法：建立HPLC—MS分析方法，分析条件为：ZorbaxSB—C18柱（4．6mm×250mm，5μm），0．5％（v／v）醋酸水溶液-0．5％（v／v）醋酸乙腈溶液为流动相，线性梯度洗脱：0min，流动相比例为95：5；25min，77：23；35min，75：25；45min，75：25；50min，70：30；75rain，10：90。流速0．5mL·min^-1，柱温35℃，检测波长254nm；负离子模式扫描，扫描范围100—1000U，氮气流速10L·min^-1，雾化温度350℃，毛细管电压3500V，裂解电压100V。依据色谱保留时间、紫外光谱及质谱信息，对丹参与丹皮配伍煎液中主要化学成分进行鉴定和归属，并比较配伍前后含量的变化。结果：鉴定并归属丹参与丹皮配伍煎液中19个成分，其中11个来自丹参，8个来自丹皮；配伍后丹酚酸类、芍药苷类成分含量高于单煎，而鞣质类成分含量降低。结论：丹参与丹皮配伍后有效成分含量得到提高。

英文摘要：

Objective：To study the chemical constituents of compatibility of Radix Salviae Mihiorrhizae and Cortex Moutan. Methods ：An HPLC- MS analytical method was developed on a Zorbax SB -ClS column （4. 6 mm × 250 mm,5 μm）with 0. 5% HAc- H20 and 0. 5%HAc- CH3CN as mobile phase. A gradient programmer was adopted according to the following profile：0 min,95：5 ;25 min,77：23 ;35 min,75：25 ;45 min,75：25 ;50 min,70：30;75 min,10： 90. The flow rate was 0. 5 mL·min^-1 with UV detected at 254 nm. The column temperature was maintained at 35℃. And the conditions for the ESI interface were as follows：negative ionization mode;drying gas flow rate, 10 L·min^-1 ;the temperature of drying gas,350℃ ;capillary voltage,3500 V;fragment voltage, 100 V. Full scan data acquisition was performed from m/z 100 to 1000 in MS scan mode. According to the information of retention time, ultraviolet spectra and mass spectra, the main constituents in combined -decoction were identified and attributed, and the content of chemical constituents in combined - decoction was compared with that in decoction individually. Results：Nineteen constituents were identified and attributed, among them eleven from Radix Salviae Miltiorrhizae and eight from Cortex Moutan. The content of phenolic acids and monoterpenoids in combined - decoction was higher than those in individual decoction ,but the content of tannins was lower. Conclusion：The results indicated that the content of some effective constituents increased after compatibility.