中文摘要：

目的 研究心复康含药血清对骨髓干细胞（BMSCs）中间质源性细胞因子α（SDF-1α）mRNA表达和蛋白分泌的影响.方法 采用全骨髓培养法分离和扩增BMSCs,并用流式细胞仪进行鉴定.以定量PCR（q-PCR）和酶联免疫吸附测定法（ELISA）分别检测含药血清作用后BMSCs中SDF-1α mRNA表达和蛋白分泌的改变情况.结果 经心复康含药血清作用72 h后,SDF-1α mRNA的表达量明显增加,实验组约为对照组的200倍（P＜0.05）;SDF-1α蛋白分泌量增加近1倍（P＜0.05）,其中实验组为（277.561 1±15.651 8）pg/ml,对照组（153.107 1±14.765 1）pg/ml.结论 全骨髓培养法可获得高纯度的BMSCs,含药血清可明显促进SDF-1αmRNA的表达及其蛋白分泌.

英文摘要：

Objective To clarify the effects of Xinfukang containing-serum on stromal cell-derived factor-1α （SDF-1α） translation and protein secretion of bone marrow stem cells （BMSCs）.Methods BMSCs were isolated and amplified using bone marrow culture method,and were identified by flow cytometry.mRNA and protein secretion of SDF-1α were detected by quantitative PCR （q-PCR） and enzyme linked immunosorbent assay （ELISA）,respectively.Results The expression of SDF-1α mRNA were significantly increased after 72 h in drug-containing serum,and SDF-1α mRNA in the experimental group was approximately 200 times as that in the control group （P＜0.05）.Secretion of SDF-1 α in the experimental group （277.561 1 ± 15.651 8） pg/ml was nearly doubled compared with that in the control group （153.107 1±14.765 1） pg/ml （P＜0.05）.Conclusions BMSCs from whole bone marrow adherent culture have high purity,and drug-containing serum can promote BMSCs to express SDF-1 α mRNA and secretion of SDF-1 α.