中文摘要：

目的：复制实验性自身免疫性重症肌无力模型大鼠，观察中药黄芪复方干预后其神经肌肉接头超微结构的变化。方法：实验于2001-01／2004-12在辽宁中医药大学附属医院完成。①选取雌性Lewis大鼠24只，随机数字表法分为模型组、正常组、中药组、西药组，6只／组。②电鳐购于美国加州，捕捞后取出电器官空运，-60℃冻存。用冷磷酸盐缓冲液将电鳐的电器官制成匀浆，制备粗提物，亲和层析技术分离提纯乙酰胆碱受体用于大鼠免疫。③除正常组外，其余组均将含有30μg乙酰胆碱受体的弗氏完全佐剂200μL注射于鼠两后足垫皮内进行初次免疫，3周后行二次免疫，将含有20μg乙酰胆碱受体的弗氏完全佐剂200μL注射于鼠两前足垫皮内，建立实验性自身免疫性重症肌无力动物模型。④初次免疫后，观察各组大鼠肌无力症状（叫声低微与活动减少是判断实验性自身免疫性重症肌无力大鼠的首发症状），并根据Lennon标准进行评分（分为0－3分．分数越高代表症状越严重）。肌电图的改变亦是判断模型建妒的重要指标，故采用肌电图检测重症肌无力衰减程度，以（第5个反应波幅-第1个反应波幅）／第1个反应波幅×100％表示。⑤成模后给予药物治疗。中药组灌胃给予自拟黄芪复方（黄芪、白术、枸杞、何首乌、枳壳、升麻）8．4g／（kg·d）；西药组灌胃给予强的松片5．4mg／（kg·d）；正常组及模型组均灌胃给予等体积生理盐水。各组给药1次／d，连续1个月。处死大鼠．电镜下观察神经肌肉接头超微结构的变化。结果：模型组2只、西药组1只大鼠因肌无力症状恶化于初次免疫后第3周死亡脱落，共21只大鼠进入结果分析。①肌无力症状Lennon标准检测结果：模型组、中药组、西药组大鼠于初次免疫后17d开始相继出现体质量下降、活动减少，叫声低微等肌无?

英文摘要：

AIM： To reproduce experimental autoimmune myasthenia gravis （EAMG） model in rats and evaluate the effects of Huangqi compound intervention on uhrastrncture of neuromuscular junction （NMJ） in rats. METHODS： The experiment was conducted in Affiliated Hospital of Liaoning University of Traditional Chinese Medicine from January 2001 to December 2004.①Twenty-four female Lewis rats were randomly divided into four groups evenly： model group, control group, traditional medicine group and western medicine group. ②At -60℃ cryopreservation, Acetylcholine receptor （AchR） was extracted from Torpedo california electric organ by affinity chromatography for immunizations of rats.③All the rats, except control group, were firstly immunized in both hind footpads by 200μL Freund＇s complete adjuvant （FCA） containing 30 μg AchR. The second immunization was carried out after three weeks in both fore footpads with 200 μL FCA containing 20 μg AchR. The EAMG models were subsequently established.④After the first immunization, the behaviors of myasthenia gravis of the rats were observed （faint cry and reduced activity were the initial symptoms of EAMG） and graded according to the Lennon standard （scored from 0 to 3 points, and the higher scores indicated the more serious symptom）. Additionally, the clinical severity of EAMG was judged by electromyography （EMG）, expressing as （amplitude of the fifth reaction wave - amplitude of the first reaction wave）/amplitude of the first reaction wave×100%. ⑤The medicine treatment was conducted following EAMG modeling. The traditional medicine group was given daily with 8.4 g/kg Huangqi compound （astragalin, white atraetrylodes, barbary wolfberry fruit, fleeceflower root. seville orange fruit and largetrifoliolious bugbane rhizome）; The western medicine group was given with 5.4 mg/kg prednisone tablet everyday; ＂Control group and model group were given with normal saline in the same volume. Each group was treated once per day for a month cont